BRENDA - Enzyme Database

Unprocessed barley aleurone endo-beta-1,4-xylanase X-I is an active enzyme

Van Campenhout, S.; Pollet, A.; Bourgois, T.M.; Rombouts, S.; Beaugrand, J.; Gebruers, K.; De Backer, E.; Courtin, C.M.; Delcour, J.A.; Volckaert, G.; Biochem. Biophys. Res. Commun. 356, 799-804 (2007) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.2.1.8
expressed in Escherichia coli
Hordeum vulgare
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.8
61500
-
unprocessed active enzyme, SDS-PAGE
Hordeum vulgare
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.8
Hordeum vulgare
Q94G06
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.8
His Trap HP column chromatography and HiPrep 26/60 Sephacryl S-100 gel filtration
Hordeum vulgare
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.8
birchwood xylan + H2O
-
677960
Hordeum vulgare
?
-
-
-
?
3.2.1.8
oat spelt xylan + H2O
-
677960
Hordeum vulgare
?
-
-
-
?
3.2.1.8
wheat arabinoxylan + H2O
-
677960
Hordeum vulgare
?
-
-
-
?
3.2.1.8
xylohexaose + H2O
rapid cleavage
677960
Hordeum vulgare
xylotriose
xylohexaose was cleaved to produce mainly xylotriose and to a lesser extent xylobiose and xylotetraose
-
-
?
3.2.1.8
xylopentaose + H2O
rapid cleavage
677960
Hordeum vulgare
xylobiose + xylotriose
-
-
-
?
3.2.1.8
xylotetraose + H2O
low activity
677960
Hordeum vulgare
xylobiose
-
-
-
?
3.2.1.8
xylotriose + H2O
low activity
677960
Hordeum vulgare
xylobiose + xylose
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
3.2.1.8
endo-beta-1,4-xylanase
-
Hordeum vulgare
3.2.1.8
X-I
-
Hordeum vulgare
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.8
40
-
-
Hordeum vulgare
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.8
0.016
-
oat spelt xylan
in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C
Hordeum vulgare
3.2.1.8
2
-
birchwood xylan
in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C
Hordeum vulgare
3.2.1.8
47.01
-
oat spelt xylan
in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C
Hordeum vulgare
3.2.1.8
72.07
-
birchwood xylan
in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C
Hordeum vulgare
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.8
5
-
-
Hordeum vulgare
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.2.1.8
Hordeum vulgare
isoelectric focusing
-
6.5
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.8
expressed in Escherichia coli
Hordeum vulgare
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.8
61500
-
unprocessed active enzyme, SDS-PAGE
Hordeum vulgare
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.8
His Trap HP column chromatography and HiPrep 26/60 Sephacryl S-100 gel filtration
Hordeum vulgare
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.8
birchwood xylan + H2O
-
677960
Hordeum vulgare
?
-
-
-
?
3.2.1.8
oat spelt xylan + H2O
-
677960
Hordeum vulgare
?
-
-
-
?
3.2.1.8
wheat arabinoxylan + H2O
-
677960
Hordeum vulgare
?
-
-
-
?
3.2.1.8
xylohexaose + H2O
rapid cleavage
677960
Hordeum vulgare
xylotriose
xylohexaose was cleaved to produce mainly xylotriose and to a lesser extent xylobiose and xylotetraose
-
-
?
3.2.1.8
xylopentaose + H2O
rapid cleavage
677960
Hordeum vulgare
xylobiose + xylotriose
-
-
-
?
3.2.1.8
xylotetraose + H2O
low activity
677960
Hordeum vulgare
xylobiose
-
-
-
?
3.2.1.8
xylotriose + H2O
low activity
677960
Hordeum vulgare
xylobiose + xylose
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.8
40
-
-
Hordeum vulgare
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.8
0.016
-
oat spelt xylan
in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C
Hordeum vulgare
3.2.1.8
2
-
birchwood xylan
in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C
Hordeum vulgare
3.2.1.8
47.01
-
oat spelt xylan
in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C
Hordeum vulgare
3.2.1.8
72.07
-
birchwood xylan
in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C
Hordeum vulgare
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.8
5
-
-
Hordeum vulgare
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.2.1.8
Hordeum vulgare
isoelectric focusing
-
6.5