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Literature summary extracted from

  • Van Campenhout, S.; Pollet, A.; Bourgois, T.M.; Rombouts, S.; Beaugrand, J.; Gebruers, K.; De Backer, E.; Courtin, C.M.; Delcour, J.A.; Volckaert, G.
    Unprocessed barley aleurone endo-beta-1,4-xylanase X-I is an active enzyme (2007), Biochem. Biophys. Res. Commun., 356, 799-804.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 expressed in Escherichia coli Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 61500
-
 unprocessed active enzyme, SDS-PAGE Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Hordeum vulgare Q94G06
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 His Trap HP column chromatography and HiPrep 26/60 Sephacryl S-100 gel filtration Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 birchwood xylan + H2O
-
 Hordeum vulgare ?
-
 ?
3.2.1.8 oat spelt xylan + H2O
-
 Hordeum vulgare ?
-
 ?
3.2.1.8 wheat arabinoxylan + H2O
-
 Hordeum vulgare ?
-
 ?
3.2.1.8 xylohexaose + H2O rapid cleavage Hordeum vulgare 2 xylotriose xylohexaose was cleaved to produce mainly xylotriose and to a lesser extent xylobiose and xylotetraose ?
3.2.1.8 xylopentaose + H2O rapid cleavage Hordeum vulgare xylobiose + xylotriose
-
 ?
3.2.1.8 xylotetraose + H2O low activity Hordeum vulgare 2 xylobiose
-
 ?
3.2.1.8 xylotriose + H2O low activity Hordeum vulgare xylobiose + xylose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 endo-beta-1,4-xylanase
-
 Hordeum vulgare
3.2.1.8 X-I
-
 Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 40
-
-
 Hordeum vulgare

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 0.016
-
 oat spelt xylan in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C Hordeum vulgare
3.2.1.8 2
-
 birchwood xylan in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C Hordeum vulgare
3.2.1.8 47.01
-
 oat spelt xylan in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C Hordeum vulgare
3.2.1.8 72.07
-
 birchwood xylan in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5
-
-
 Hordeum vulgare

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Hordeum vulgare isoelectric focusing
-
 6.5