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Literature summary extracted from

  • Toyama, H.; Nishibayashi, E.; Saeki, M.; Adachi, O.; Matsushita, K.
    Factors required for the catalytic reaction of PqqC/D which produces pyrroloquinoline quinone (2007), Biochem. Biophys. Res. Commun., 354, 290-295.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.3.11 activating factor ActF consists of a cytosolic fraction, activating factor ActF enhances pyrroloquinoline quinone synthesis in the presence of NAD(P)H Escherichia coli
1.3.3.11 activating factor ActF consists of a cytosolic fraction, activating factor ActF enhances pyrroloquinoline quinone synthesis in the presence of NAD(P)H Methylorubrum extorquens
1.3.3.11 dithioerythritol
-
 Klebsiella pneumoniae
1.3.3.11 dithioerythritol
-
 Methylorubrum extorquens
1.3.3.11 dithiothreitol
-
 Klebsiella pneumoniae
1.3.3.11 dithiothreitol
-
 Methylorubrum extorquens
1.3.3.11 dithiothreitol 0.1 mM enhances pyrroloquinoline quinone production Escherichia coli
1.3.3.11 dithiothreitol 0.1 mM enhances pyrroloquinoline quinone production Methylorubrum extorquens
1.3.3.11 NAD(P)H NAD(P)H enhances pyrroloquinoline quinone production in the presence of activating factor ActF Escherichia coli
1.3.3.11 NAD(P)H NAD(P)H enhances pyrroloquinoline quinone production in the presence of activating factor ActF Methylorubrum extorquens
1.3.3.11 TrxAB the complete TrxAB system enhance the pyrroloquinoline quinone production Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.11
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.11 oxidized pyrroloquinoline quinone in vitro pyrroloquinoline quinone is oxidized by molecular oxygen and then the oxidized pyrroloquinoline quinone is trapped in PqqC/D to show product inhibition Escherichia coli
1.3.3.11 oxidized pyrroloquinoline quinone in vitro pyrroloquinoline quinone is oxidized by molecular oxygen and then the oxidized pyrroloquinoline quinone is trapped in PqqC/D to show product inhibition Methylorubrum extorquens
1.3.3.11 pyrroloquinoline quinone
-
 Escherichia coli
1.3.3.11 pyrroloquinoline quinone
-
 Klebsiella pneumoniae
1.3.3.11 pyrroloquinoline quinone
-
 Methylorubrum extorquens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.11 29000
-
 2 * 29000, SDS-PAGE Escherichia coli
1.3.3.11 29000
-
 2 * 29000, SDS-PAGE Methylorubrum extorquens
1.3.3.11 58000
-
 SDS-PAGE Escherichia coli
1.3.3.11 58000
-
 SDS-PAGE Methylorubrum extorquens
1.3.3.11 150000
-
 determined by gel filtration, with DTT Methylorubrum extorquens
1.3.3.11 300000
-
 determined by gel filtration, without DTT Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.11 6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,5,6,7,8,-octahydroquinoline-2,4-dicarboxylate + O2 Escherichia coli
-
 4,5-dioxo-3a,4,5,6,7,8,9,9b-octahydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
1.3.3.11 6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,5,6,7,8,-octahydroquinoline-2,4-dicarboxylate + O2 Klebsiella pneumoniae
-
 4,5-dioxo-3a,4,5,6,7,8,9,9b-octahydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
1.3.3.11 6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,5,6,7,8,-octahydroquinoline-2,4-dicarboxylate + O2 Methylorubrum extorquens
-
 4,5-dioxo-3a,4,5,6,7,8,9,9b-octahydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
1.3.3.11 6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,5,6,7,8,-octahydroquinoline-2,4-dicarboxylate + O2 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
 4,5-dioxo-3a,4,5,6,7,8,9,9b-octahydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
1.3.3.11 additional information Methylorubrum extorquens PqqC/D produces the reduced pyrroloquinoline quinone from the intermediate in vivo ?
-
 ?
1.3.3.11 additional information Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 PqqC/D produces the reduced pyrroloquinoline quinone from the intermediate in vivo ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.11 Escherichia coli
-
-
-
1.3.3.11 Klebsiella pneumoniae
-
-
-
1.3.3.11 Methylorubrum extorquens
-
-
-
1.3.3.11 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.11
-
 Escherichia coli
1.3.3.11
-
 Methylorubrum extorquens
1.3.3.11 Superdex S-200 gel filtration Escherichia coli
1.3.3.11 Superdex S-200 gel filtration Methylorubrum extorquens

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.3.11 6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,7,8-hexahydroquinoline-2,4-dicarboxylate + 3 O2 = 4,5-dioxo-4,5-dihydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + 2 H2O2 + 2 H2O PqqC/D produces the reduced pyrroloquinoline quinone from the intermediate in vivo Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.11 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2
-
 Escherichia coli 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
1.3.3.11 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2
-
 Methylorubrum extorquens 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
1.3.3.11 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2
-
 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
1.3.3.11 6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,5,6,7,8,-octahydroquinoline-2,4-dicarboxylate + O2
-
 Escherichia coli 4,5-dioxo-3a,4,5,6,7,8,9,9b-octahydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
1.3.3.11 6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,5,6,7,8,-octahydroquinoline-2,4-dicarboxylate + O2
-
 Klebsiella pneumoniae 4,5-dioxo-3a,4,5,6,7,8,9,9b-octahydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
1.3.3.11 6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,5,6,7,8,-octahydroquinoline-2,4-dicarboxylate + O2
-
 Methylorubrum extorquens 4,5-dioxo-3a,4,5,6,7,8,9,9b-octahydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
1.3.3.11 6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,5,6,7,8,-octahydroquinoline-2,4-dicarboxylate + O2
-
 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 4,5-dioxo-3a,4,5,6,7,8,9,9b-octahydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
1.3.3.11 additional information PqqC/D produces the reduced pyrroloquinoline quinone from the intermediate in vivo Methylorubrum extorquens ?
-
 ?
1.3.3.11 additional information PqqC/D has low affinity to PQQH2 Escherichia coli ?
-
 ?
1.3.3.11 additional information PqqC/D has low affinity to PQQH2 Methylorubrum extorquens ?
-
 ?
1.3.3.11 additional information PqqC/D produces the reduced pyrroloquinoline quinone from the intermediate in vivo Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 ?
-
 ?
1.3.3.11 additional information PqqC/D has low affinity to PQQH2 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.11 homodimer 2 * 29000, SDS-PAGE Escherichia coli
1.3.3.11 homodimer 2 * 29000, SDS-PAGE Methylorubrum extorquens

Synonyms

EC Number Synonyms Comment Organism
1.3.3.11 PqqC
-
 Escherichia coli
1.3.3.11 PqqC
-
 Klebsiella pneumoniae
1.3.3.11 PqqC
-
 Methylorubrum extorquens
1.3.3.11 PqqC/D
-
 Escherichia coli
1.3.3.11 PqqC/D
-
 Klebsiella pneumoniae
1.3.3.11 PqqC/D
-
 Methylorubrum extorquens
1.3.3.11 pyrroloquinoline-quinone synthase
-
 Escherichia coli
1.3.3.11 pyrroloquinoline-quinone synthase
-
 Klebsiella pneumoniae
1.3.3.11 pyrroloquinoline-quinone synthase
-
 Methylorubrum extorquens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.3.11 25
-
 activity assay Escherichia coli
1.3.3.11 25
-
 activity assay Klebsiella pneumoniae
1.3.3.11 25
-
 activity assay Methylorubrum extorquens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.11 8.3
-
 activity assay Escherichia coli
1.3.3.11 8.3
-
 activity assay Klebsiella pneumoniae
1.3.3.11 8.3
-
 activity assay Methylorubrum extorquens