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Literature summary extracted from
- Degradation of cellulosome-produced cello-oligosaccharides by an extracellular non-cellulosomal beta-glucan glucohydrolase, BglA, from Clostridium cellulovorans (2006), Biochem. Biophys. Res. Commun., 349, 20-23.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.74 | expression in Escherichia coli | Clostridium cellulovorans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.74 | D-glucose | at 20 mM | Clostridium cellulovorans |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.74 | 1.1 | - |
cellotetraose | - |
Clostridium cellulovorans | |
| 3.2.1.74 | 1.7 | - |
cellotriose | - |
Clostridium cellulovorans | |
| 3.2.1.74 | 2.9 | - |
cellobiose | - |
Clostridium cellulovorans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 51570 | - |
calculated | Clostridium cellulovorans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.74 | Clostridium cellulovorans | Q53EH2 | ATCC35296 | - |
| 3.2.1.74 | Clostridium cellulovorans ATCC 35296 | Q53EH2 | ATCC35296 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.74 | Ni-nitrilotriacetatic acid agarose column chromatography | Clostridium cellulovorans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 129 | - |
p-nitrophenyl-beta-D-fucopyranoside as substrate | Clostridium cellulovorans |
| 3.2.1.74 | 130 | - |
o-nitrophenyl-beta-D-galactopyranoside as substrate | Clostridium cellulovorans |
| 3.2.1.74 | 130 | - |
p-nitrophenyl-beta-D-cellobioside as substrate | Clostridium cellulovorans |
| 3.2.1.74 | 133 | - |
p-nitrophenyl-beta-D-galactopyranoside as substrate | Clostridium cellulovorans |
| 3.2.1.74 | 177 | - |
p-nitrophenyl-beta-D-glucopyranoside as substrate | Clostridium cellulovorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.74 | 4-nitrophenyl beta-D-glucopyranose + H2O | - |
Clostridium cellulovorans | 4-nitrophenol + D-glucose | - |
? | |
| 3.2.1.74 | cellobiose + H2O | - |
Clostridium cellulovorans | 2 D-glucose | - |
? | |
| 3.2.1.74 | cellobiose + H2O | - |
Clostridium cellulovorans ATCC 35296 | 2 D-glucose | - |
? | |
| 3.2.1.74 | cellooligomers + H2O | - |
Clostridium cellulovorans | D-glucose + ? | - |
? | |
| 3.2.1.74 | cellooligomers + H2O | - |
Clostridium cellulovorans ATCC 35296 | D-glucose + ? | - |
? | |
| 3.2.1.74 | cellotetraose + 3 H2O | - |
Clostridium cellulovorans | 4 D-glucose | - |
? | |
| 3.2.1.74 | cellotetraose + 3 H2O | - |
Clostridium cellulovorans ATCC 35296 | 4 D-glucose | - |
? | |
| 3.2.1.74 | cellotriose + H2O | - |
Clostridium cellulovorans | D-glucose + cellobiose | - |
? | |
| 3.2.1.74 | cellotriose + H2O | - |
Clostridium cellulovorans ATCC 35296 | D-glucose + cellobiose | - |
? | |
| 3.2.1.74 | o-nitrophenyl-beta-D-galactopyranose + H2O | - |
Clostridium cellulovorans | o-nitrophenol + beta-D-galactopyranose | - |
? | |
| 3.2.1.74 | o-nitrophenyl-beta-D-galactopyranose + H2O | - |
Clostridium cellulovorans ATCC 35296 | o-nitrophenol + beta-D-galactopyranose | - |
? | |
| 3.2.1.74 | p-nitrophenyl-beta-D-cellobiose + H2O | - |
Clostridium cellulovorans | p-nitrophenol + cellobioside | - |
? | |
| 3.2.1.74 | p-nitrophenyl-beta-D-fucopyranoside + H2O | - |
Clostridium cellulovorans | p-nitrophenol + fucopyranose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.74 | b-glucan glucohydrolase | extracellular non-cellulosomal beta-glucan glucohydrolase | Clostridium cellulovorans |
| 3.2.1.74 | BglA | - |
Clostridium cellulovorans |
| 3.2.1.74 | family-1 glycosyl hydrolase | - |
Clostridium cellulovorans |
| 3.2.1.74 | glucan 1,4-beta-glucosidase | - |
Clostridium cellulovorans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 50 | - |
- |
Clostridium cellulovorans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 6 | - |
- |
Clostridium cellulovorans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 5 | 8 | - |
Clostridium cellulovorans |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 5 | 8 | when incubated at 40°C for 12 h with no substrate | Clostridium cellulovorans |
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Release 2023.1 (January 2023)
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