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Literature summary extracted from

  • Phillips, R.S.; McPhie, P.; Miles, E.W.; Marchal, S.; Lange, R.
    Quantitative effects of allosteric ligands and mutations on conformational equilibria in Salmonella typhimurium tryptophan synthase (2008), Arch. Biochem. Biophys., 470, 8-19.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.20 expressed in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.20 D305A much broader substrate specificity for nucleophiles than the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 E109D modest but significant effects of 40 to 2000fold on the conformational equilibrium Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.20 Cs+ has an effect on the conformational equilibrium Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 K+ has an effect on the conformational equilibrium Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 Li+ has an effect on the conformational equilibrium Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 Na+ has an effect on the conformational equilibrium Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 NH4+ has little effect on the conformational equilibrium Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 Rb+ has an effect on the conformational equilibrium Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.20 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.20 PD-10 column chromatography, desalting Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.20 L-serine + indole
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.20 Trp synthase
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.20 pyridoxal 5'-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium