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Literature summary extracted from

  • Aggarwal, M.; Kishan, K.V.; Mondal, A.K.
    Creation of salt-insensitive 3(2),5-bisphosphate nucleotidase by modeling and mutagenesis approach (2008), Arch. Biochem. Biophys., 469, 174-183.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.7 expressed in Escherichia coli BL21 (DE3) cells Debaryomyces hansenii

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.7 D201N 8.2% relative activity compared to the wild type enzyme Debaryomyces hansenii
3.1.3.7 D363N 6.2% relative activity compared to the wild type enzyme Debaryomyces hansenii
3.1.3.7 E133Q 4.8% relative activity compared to the wild type enzyme Debaryomyces hansenii
3.1.3.7 E301K strongly reduced activity compared to the wild type enzyme Debaryomyces hansenii
3.1.3.7 T206A 5.6% relative activity compared to the wild type enzyme Debaryomyces hansenii
3.1.3.7 V131A strongly reduced activity compared to the wild type enzyme Debaryomyces hansenii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.7 Li+
-
Debaryomyces hansenii
3.1.3.7 Na+
-
Debaryomyces hansenii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.7 0.01704
-
adenosine 3',5'-bisphosphate wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii
3.1.3.7 0.02646
-
adenosine 3',5'-bisphosphate mutant enzyme E301K, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii
3.1.3.7 0.07299
-
adenosine 3',5'-bisphosphate mutant enzyme V131A, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.7 Debaryomyces hansenii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.7 Ni-NTA column chromatography Debaryomyces hansenii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.7 adenosine 3',5'-bisphosphate + H2O
-
Debaryomyces hansenii adenosine 5'-phosphate + phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.7 3(2),5-bisphosphate nucleotidase
-
Debaryomyces hansenii
3.1.3.7 BPntase
-
Debaryomyces hansenii
3.1.3.7 Dhal2p
-
Debaryomyces hansenii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.7 0.069
-
adenosine 3',5'-bisphosphate mutant enzyme E301K, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii
3.1.3.7 1.465
-
adenosine 3',5'-bisphosphate mutant enzyme V131A, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii
3.1.3.7 1.921
-
adenosine 3',5'-bisphosphate wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii
3.1.3.7 2.94
-
adenosine 3',5'-bisphosphate wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii
3.1.3.7 6.08
-
adenosine 3',5'-bisphosphate mutant enzyme V131A, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.7 2.4
-
wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii Li+
3.1.3.7 180
-
wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C Debaryomyces hansenii Na+