EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.7 | expressed in Escherichia coli BL21 (DE3) cells | Debaryomyces hansenii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.7 | D201N | 8.2% relative activity compared to the wild type enzyme | Debaryomyces hansenii |
3.1.3.7 | D363N | 6.2% relative activity compared to the wild type enzyme | Debaryomyces hansenii |
3.1.3.7 | E133Q | 4.8% relative activity compared to the wild type enzyme | Debaryomyces hansenii |
3.1.3.7 | E301K | strongly reduced activity compared to the wild type enzyme | Debaryomyces hansenii |
3.1.3.7 | T206A | 5.6% relative activity compared to the wild type enzyme | Debaryomyces hansenii |
3.1.3.7 | V131A | strongly reduced activity compared to the wild type enzyme | Debaryomyces hansenii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.7 | Li+ | - |
Debaryomyces hansenii | |
3.1.3.7 | Na+ | - |
Debaryomyces hansenii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.7 | 0.01704 | - |
adenosine 3',5'-bisphosphate | wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii | |
3.1.3.7 | 0.02646 | - |
adenosine 3',5'-bisphosphate | mutant enzyme E301K, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii | |
3.1.3.7 | 0.07299 | - |
adenosine 3',5'-bisphosphate | mutant enzyme V131A, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.7 | Debaryomyces hansenii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.7 | Ni-NTA column chromatography | Debaryomyces hansenii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.7 | adenosine 3',5'-bisphosphate + H2O | - |
Debaryomyces hansenii | adenosine 5'-phosphate + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.7 | 3(2),5-bisphosphate nucleotidase | - |
Debaryomyces hansenii |
3.1.3.7 | BPntase | - |
Debaryomyces hansenii |
3.1.3.7 | Dhal2p | - |
Debaryomyces hansenii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.7 | 0.069 | - |
adenosine 3',5'-bisphosphate | mutant enzyme E301K, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii | |
3.1.3.7 | 1.465 | - |
adenosine 3',5'-bisphosphate | mutant enzyme V131A, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii | |
3.1.3.7 | 1.921 | - |
adenosine 3',5'-bisphosphate | wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii | |
3.1.3.7 | 2.94 | - |
adenosine 3',5'-bisphosphate | wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii | |
3.1.3.7 | 6.08 | - |
adenosine 3',5'-bisphosphate | mutant enzyme V131A, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.1.3.7 | 2.4 | - |
wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii | Li+ | |
3.1.3.7 | 180 | - |
wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30°C | Debaryomyces hansenii | Na+ |