Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhang, A.; Liu, W.F.; Yan, Y.B.
    Role of the RRM domain in the activity, structure and stability of poly(A)-specific ribonuclease (2007), Arch. Biochem. Biophys., 461, 255-262.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.13.4 additional information the entire RRM domain not only contributes to the substrate binding and efficient catalysis of PARN, but also stabilizes the overall structures of the protein. p46 contains only one Trp residue (W219), p54 contains two (W219 and W456), and p74 contains six (W219, W456, W474, W526, W531 and W639) Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.13.4 into vector pET28a, expressed in Escherichia coli BL21(DE3)pLys(S) cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.13.4 guanidine hydrochloride full-length enzyme retains most of its activity at guanidine hydrochloride concentrations below 0.5 M, whereas an abrupt decrease of the activity of p54 and p46 is found when guanidine hydrochloride concentration is increased Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.13.4 0.0000236
-
 poly(A) RNA full-length 74 kDa PARN Homo sapiens
3.1.13.4 0.0000303
-
 poly(A) RNA 54 kDa form Homo sapiens
3.1.13.4 0.0000333
-
 poly(A) RNA 46 kDa form Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.13.4 46000
-
 truncated form completely missing the RRM Homo sapiens
3.1.13.4 54000
-
 proteolytic fragment with half of the RRM Homo sapiens
3.1.13.4 74000
-
 full-length PARN Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.13.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.13.4 on Ni-NTA resin and by gel filtration, purity above 98% Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.13.4 poly(A) RNA + H2O
-
 Homo sapiens 5'-AMP + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.13.4 PARN
-
 Homo sapiens