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Literature summary extracted from

  • Olczak, M.; Olczak, T.
    N-glycosylation sites of plant purple acid phosphatases important for protein expression and secretion in insect cells (2007), Arch. Biochem. Biophys., 461, 247-254.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.2 expressed in Sf9 TriEx insect cells Phaseolus vulgaris
3.1.3.2 expressed in Sf9 TriEx insect cells Lupinus luteus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.2 K301N activity comparable to the wild type enzyme Phaseolus vulgaris
3.1.3.2 N105S inactive Lupinus luteus
3.1.3.2 N105S/N367S inactive Lupinus luteus
3.1.3.2 N105S/N367S/N424S inactive Lupinus luteus
3.1.3.2 N105S/N424S inactive Lupinus luteus
3.1.3.2 N108S inactive Phaseolus vulgaris
3.1.3.2 N108S/N170S inactive Phaseolus vulgaris
3.1.3.2 N108S/N423S inactive Phaseolus vulgaris
3.1.3.2 N108SS/N170S/N423S inactive Phaseolus vulgaris
3.1.3.2 N170S inactive Phaseolus vulgaris
3.1.3.2 N170S/N423S inactive Phaseolus vulgaris
3.1.3.2 N367S inactive Lupinus luteus
3.1.3.2 N367S/N424S inactive Lupinus luteus
3.1.3.2 N423S inactive Phaseolus vulgaris
3.1.3.2 N424S inactive Lupinus luteus
3.1.3.2 S110A reduced activity Phaseolus vulgaris
3.1.3.2 S110P reduced activity Phaseolus vulgaris
3.1.3.2 S110T activity comparable to the wild type enzyme Phaseolus vulgaris
3.1.3.2 T107I reduced activity Lupinus luteus
3.1.3.2 T107N reduced activity Lupinus luteus
3.1.3.2 T107S activity comparable to the wild type enzyme Lupinus luteus
3.1.3.2 T172A inactive Phaseolus vulgaris
3.1.3.2 T172S activity comparable to the wild type enzyme Phaseolus vulgaris
3.1.3.2 T369A activity comparable to the wild type enzyme Lupinus luteus
3.1.3.2 T369P activity comparable to the wild type enzyme Lupinus luteus
3.1.3.2 T369S activity comparable to the wild type enzyme Lupinus luteus
3.1.3.2 T425A inactive Phaseolus vulgaris
3.1.3.2 T425P inactive Phaseolus vulgaris
3.1.3.2 T425S activity comparable to the wild type enzyme Phaseolus vulgaris
3.1.3.2 T426I reduced activity Lupinus luteus
3.1.3.2 T426N reduced activity Lupinus luteus
3.1.3.2 T426S activity comparable to the wild type enzyme Lupinus luteus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Lupinus luteus
-
-
-
3.1.3.2 Phaseolus vulgaris
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.2 glycoprotein
-
 Phaseolus vulgaris
3.1.3.2 glycoprotein
-
 Lupinus luteus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 leaf
-
 Phaseolus vulgaris
-
3.1.3.2 leaf
-
 Lupinus luteus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 ATP + H2O low affinity to ATP Lupinus luteus ADP + phosphate
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Phaseolus vulgaris p-nitrophenol + phosphate
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Lupinus luteus p-nitrophenol + phosphate
-
 ?
3.1.3.2 phosphoenolpyruvate + H2O prefers phosphoenolpyruvate as a substrate Lupinus luteus pyruvate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 PAP
-
 Phaseolus vulgaris
3.1.3.2 PAP
-
 Lupinus luteus
3.1.3.2 purple acid phosphatase
-
 Phaseolus vulgaris
3.1.3.2 purple acid phosphatase
-
 Lupinus luteus