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Literature summary extracted from

  • Seidle, H.F.; Allison, S.J.; George, E.; Huber, R.E.
    Trp-49 of the family 3 beta-glucosidase from Aspergillus niger is important for its transglucosidic activity: creation of novel beta-glucosidases with low transglucosidic efficiencies (2006), Arch. Biochem. Biophys., 455, 110-118.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 methanol-inducible expression of wild-type and mutant enzymes in Pichia pastoris strain X-33 Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.21 W49A site-directed mutagenesis, the mutation reduces the transglucosylation activity of the enzyme due to a large decrease in binding capacity for Glc at site +1 and an increase in binding ability at site-1, while the hydrolytic activity is only slightly decreased compared to the wild-type enzyme, the mutant shows altered kinetics, overview Aspergillus niger
3.2.1.21 W49D site-directed mutagenesis, the mutation reduces the transglucosylation activity of the enzyme due to a large decrease in binding capacity for Glc at site +1 and an increase in binding ability at site-1, while the hydrolytic activity is only slightly decreased compared to the wild-type enzyme, the mutant shows altered kinetics, overview Aspergillus niger
3.2.1.21 W49G site-directed mutagenesis, the mutation reduces the transglucosylation activity of the enzyme due to a large decrease in binding capacity for Glc at site +1 and an increase in binding ability at site-1, while the hydrolytic activity is only slightly decreased compared to the wild-type enzyme, the mutant shows altered kinetics, overview Aspergillus niger
3.2.1.21 W49N site-directed mutagenesis, the mutation reduces the transglucosylation activity of the enzyme due to a large decrease in binding capacity for Glc at site +1 and an increase in binding ability at site-1, while the hydrolytic activity is only slightly decreased compared to the wild-type enzyme, the mutant shows altered kinetics, overview Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 cellobiose competitive inhibition of wild-type and mutant enzymes Aspergillus niger
3.2.1.21 D-gluconolactone competitive inhibition of wild-type enzyme and mutant W49A Aspergillus niger
3.2.1.21 D-glucose competitive inhibition of wild-type and mutant enzymes Aspergillus niger
3.2.1.21 gentiobiose competitive inhibition of wild-type enzyme and mutant W49A Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 additional information
-
 additional information kinetics of hydrolytic activity of mutant enzymes and of transglucosylation activities of recombinant wild-type and mutant enzymes, overview Aspergillus niger
3.2.1.21 0.27
-
 cellotetraose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
3.2.1.21 0.33
-
 cellopentaose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
3.2.1.21 0.52
-
 cellotriose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
3.2.1.21 1.5
-
 4-nitrophenyl-beta-D-glucopyranoside pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
3.2.1.21 2.2
-
 gentiobiose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
3.2.1.21 2.7
-
 cellobiose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 cellobiose + H2O Aspergillus niger
-
 2 beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Aspergillus niger
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.21 celloheptaose + 6 H2O = 7 beta-D-glucose Trp49 is important for transglucosylation catalytic activity Aspergillus niger
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus niger 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Aspergillus niger 2 beta-D-glucose
-
 ?
3.2.1.21 cellopentaose + H2O
-
 Aspergillus niger 5 D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O
-
 Aspergillus niger 4 beta-D-glucose
-
 ?
3.2.1.21 cellotriose + H2O
-
 Aspergillus niger 3 D-glucose
-
 ?
3.2.1.21 gentiobiose + H2O
-
 Aspergillus niger 2 beta-D-glucose
-
 ?
3.2.1.21 additional information the enzyme shows transglucosylation activity involving Trp49, overview Aspergillus niger ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 More the enzyme belongs to the glycosyl hydrolase family 3 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 25
-
 assay at Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 2 3.7 cellotriose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
3.2.1.21 51.7
-
 cellopentaose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
3.2.1.21 54.7
-
 cellotetraose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
3.2.1.21 70
-
 gentiobiose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
3.2.1.21 85.1
-
 cellotriose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
3.2.1.21 89
-
 4-nitrophenyl-beta-D-glucopyranoside pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
3.2.1.21 103
-
 cellobiose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 4.5
-
 assay at Aspergillus niger

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.21 0.2
-
 D-gluconolactone pH 4.5, 25°C, recombinant mutant W49A Aspergillus niger
3.2.1.21 0.4
-
 D-gluconolactone pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
3.2.1.21 1.1
-
 cellobiose pH 4.5, 25°C, recombinant mutant W49D Aspergillus niger
3.2.1.21 1.2
-
 cellobiose pH 4.5, 25°C, recombinant mutant W49N Aspergillus niger
3.2.1.21 1.3
-
 cellobiose pH 4.5, 25°C, recombinant mutant W49G Aspergillus niger
3.2.1.21 1.4
-
 cellobiose pH 4.5, 25°C, recombinant mutant W49A Aspergillus niger
3.2.1.21 1.6
-
 cellobiose pH 4.5, 25°C, recombinant mutants W49N and W49G Aspergillus niger
3.2.1.21 1.8
-
 gentiobiose pH 4.5, 25°C, recombinant mutant W49A Aspergillus niger
3.2.1.21 1.8
-
 cellobiose pH 4.5, 25°C, recombinant wild-type enzyme and mutant W49A Aspergillus niger
3.2.1.21 1.9
-
 cellobiose pH 4.5, 25°C, recombinant mutant W49D Aspergillus niger
3.2.1.21 3
-
 D-glucose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
3.2.1.21 4.8
-
 gentiobiose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger