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Literature summary extracted from

  • Schepmann, H.G.; Pang, J.; Matsuda, S.P.T.
    Cloning and characterization of Ginkgo biloba levopimaradiene synthase, which catalyzes the first committed step in ginkgolide biosynthesis (2001), Arch. Biochem. Biophys., 392, 263-269.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.32 dithiothreitol optimal activity in the presence of 5 mM DTT and 5% glycerol Ginkgo biloba

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.32 expression in Escherichia coli Ginkgo biloba

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.32 additional information removal of 60 or 79 N-terminal residues increases levopimaradiene production, deletion of 128 N-terminal residues results in loss of catalytic activity Ginkgo biloba

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.32 plastid sequence includes a putative N-terminal plastid transit peptide Ginkgo biloba 9536
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.32 Mn2+ required Ginkgo biloba
4.2.3.32 additional information enzyme is not dependent on Mg2+ Ginkgo biloba

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.32 100289
-
 x * 100289, calculated Ginkgo biloba

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.32 additional information Ginkgo biloba catalyzes an intermediate step in synthesis of ginkgolide A ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.32 Ginkgo biloba Q947C4
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.3.32 additional information sequence includes a putative N-terminal plastid transit peptide and three aspartate-rich regions Ginkgo biloba

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.32 root root of seedling Ginkgo biloba
-
4.2.3.32 seedling root of seedling Ginkgo biloba
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.32 (+)-copalyl-diphosphate reaction intermediate is labdadienyl diphosphate Ginkgo biloba levopimaradiene + diphosphate
-
 ?
4.2.3.32 additional information catalyzes an intermediate step in synthesis of ginkgolide A Ginkgo biloba ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.32 ? x * 100289, calculated Ginkgo biloba

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.32 7.2
-
-
 Ginkgo biloba