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Literature summary extracted from

  • Zouari Ayadi, D.; Ben Ali, M.; Jemli, S.; Ben Mabrouk, S.; Mezghani, M.; Ben Messaoud, E.; Bejar, S.
    Heterologous expression, secretion and characterization of the Geobacillus thermoleovorans US105 type I pullulanase (2008), Appl. Microbiol. Biotechnol., 78, 473-481.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.41 expression in Escherichia coli, connection to the lipase A signal peptide of Bacillus subtilis strain leads to an efficient secretion of an active form enzyme on the periplasmic fraction, fusion to the amylase signal sequence of the Bacillus stearothermophilus US100 strain leads to an efficient secretion of this natively cytoplasmic enzyme as an active form in the supernatant Geobacillus thermoleovorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.41 cyclodextrin
-
 Geobacillus thermoleovorans
3.2.1.41 additional information EDTA has no effect Geobacillus thermoleovorans
3.2.1.41 SDS
-
 Geobacillus thermoleovorans
3.2.1.41 Triton X-100
-
 Geobacillus thermoleovorans
3.2.1.41 Urea
-
 Geobacillus thermoleovorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.41 Ca2+ stabilizes the enzyme at temperatures above 45°C Geobacillus thermoleovorans
3.2.1.41 Co2+ inhibitory Geobacillus thermoleovorans
3.2.1.41 Cu2+ complete inhibitory Geobacillus thermoleovorans
3.2.1.41 Fe2+ inhibitory Geobacillus thermoleovorans
3.2.1.41 Mn2+ complete inhibitory Geobacillus thermoleovorans
3.2.1.41 additional information Mg2+, Zn2+ have no activity Geobacillus thermoleovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 80000
-
 2 * 80000 Geobacillus thermoleovorans
3.2.1.41 160000
-
 SDS-PAGE Geobacillus thermoleovorans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Geobacillus thermoleovorans
-
 previously B. thermoleovorans US105
-
3.2.1.41 Geobacillus thermoleovorans US105
-
 previously B. thermoleovorans US105
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41 of the recombinant protein using heat treatment, size exclusion, and anion-exchange chromatography Geobacillus thermoleovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.41 3.2
-
 of the periplasmic homogenate before purification Geobacillus thermoleovorans
3.2.1.41 12
-
 in the supernatant Geobacillus thermoleovorans
3.2.1.41 36
-
 of the periplasmic homogenate after purification Geobacillus thermoleovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 pullulan + H2O
-
 Geobacillus thermoleovorans maltotriose + maltose
-
 ?
3.2.1.41 pullulan + H2O
-
 Geobacillus thermoleovorans US105 maltotriose + maltose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.41 dimer 2 * 80000 Geobacillus thermoleovorans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 PUL US105
-
 Geobacillus thermoleovorans
3.2.1.41 pullulanase
-
 Geobacillus thermoleovorans
3.2.1.41 pullulanase type 1
-
 Geobacillus thermoleovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 70
-
 with 1 mM CaCl2 Geobacillus thermoleovorans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.41 45 70
-
 Geobacillus thermoleovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 6
-
 with 1 mM CaCl2 Geobacillus thermoleovorans