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Literature summary extracted from

  • Saibi, W.; Amouri, B.; Gargouri, A.
    Purification and biochemical characterization of a transglucosilating beta-glucosidase of Stachybotrys strain (2007), Appl. Microbiol. Biotechnol., 77, 293-300.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Fe2+ activates at 5 mM, but shows 50% inhibition at 10 mM Stachybotrys sp.
3.2.1.21 additional information optimal activity is obtained with 100 mM phosphate buffer and, the activity decreased at 150 and 200 mM but not dramatically Stachybotrys sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 1.85
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.0, 50°C Stachybotrys sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 extracellular
-
 Stachybotrys sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.21 Ba2+ activates 1.4fold at 10 mM Stachybotrys sp.
3.2.1.21 Ca2+ activates Stachybotrys sp.
3.2.1.21 Fe2+ activates at 5 mM, but shows 50% inhibition at 10 mM Stachybotrys sp.
3.2.1.21 additional information no effect by Co2+, optimal activity is obtained with 100 mM phosphate buffer and, the activity decreased at 150 and 200 mM but not dramatically Stachybotrys sp.
3.2.1.21 Zn2+ activates Stachybotrys sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 78000
-
 gel filtration Stachybotrys sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 additional information Stachybotrys sp. the enzyme has the capacity to synthesize short oligosaccharides from cellobiose as the substrate concentration reaches 30% with a recovery of 40%, involvement of a transglucosylation to synthesize cellotetraose by a sequential addition of glucose to cellotriose, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Stachybotrys sp.
-
 a filamentous fungus, strain A19, isolated after nitrous acid mutagenesis from the cellulolytic fungus N1, five isozymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 native extracellular isozyme by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration Stachybotrys sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Stachybotrys sp. 4-methylumbelliferol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Stachybotrys sp. 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Stachybotrys sp. 2 beta-D-glucose
-
 ?
3.2.1.21 additional information the enzyme has the capacity to synthesize short oligosaccharides from cellobiose as the substrate concentration reaches 30% with a recovery of 40%, involvement of a transglucosylation to synthesize cellotetraose by a sequential addition of glucose to cellotriose, overview Stachybotrys sp. ?
-
 ?
3.2.1.21 additional information substrate specificity, overview, no activity with 4-methylumbelliferyl-beta-D-cellobioside, the enzyme has the capacity to synthesize short oligosaccharides from cellobiose as the substrate concentration reaches 30% with a recovery of 40%, overview Stachybotrys sp. ?
-
 ?
3.2.1.21 salicin + H2O
-
 Stachybotrys sp. ? + beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 78000, SDS-PAGE Stachybotrys sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 50
-
-
 Stachybotrys sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 915
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.0, 50°C Stachybotrys sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6
-
-
 Stachybotrys sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 4 10 stable Stachybotrys sp.