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Literature summary extracted from

  • Fukuda, T.; Kato-Murai, M.; Kuroda, K.; Ueda, M.; Suye, S.
    Improvement in enzymatic desizing of starched cotton cloth using yeast codisplaying glucoamylase and cellulose-binding domain (2008), Appl. Microbiol. Biotechnol., 77, 1225-1232.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.3 industry improvement in enzymatic desizing of starched cotton cloth using yeast codisplaying glucoamylase and cellulose-binding domain, overview Rhizopus arrhizus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.3 expression in Saccharomyces cerevisiae strain MT8-1 on the cell surface, subcloning in Escherichia coli Rhizopus arrhizus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.3 additional information use of cell surface engineering to display Rhizopus oryzae glucoamylase on the cell surface of yeast Saccharomyces cerevisiae, improvement in enzymatic desizing of starched cotton cloth using yeast codisplaying glucoamylase and cellulose-binding domain, overview Rhizopus arrhizus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.3 Mg2+
-
 Rhizopus arrhizus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.3 starch + H2O Rhizopus arrhizus glucoamylase is an exo-amylolytic enzyme that cleaves alpha-1,4-linked and alpha-1,6-linked glucose from starch beta-D-glucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Rhizopus arrhizus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 additional information
-
 enzyme activity of different recombinant constructs in Saccharomyces cerevisiae cultures, overview Rhizopus arrhizus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 starch + H2O
-
 Rhizopus arrhizus beta-D-glucose + ?
-
 ?
3.2.1.3 starch + H2O glucoamylase is an exo-amylolytic enzyme that cleaves alpha-1,4-linked and alpha-1,6-linked glucose from starch Rhizopus arrhizus beta-D-glucose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 glucoamylase
-
 Rhizopus arrhizus
3.2.1.3 More the enzyme belongs to the family 15 glycoside hydrolase Rhizopus arrhizus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 37
-
 assay at Rhizopus arrhizus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 6
-
 assay at Rhizopus arrhizus