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Literature summary extracted from

  • Weaver, J.D.; Mullaney, E.J.; Lei, X.G.
    Altering the substrate specificity site of Aspergillus niger PhyB shifts the pH optimum to pH 3.2 (2007), Appl. Microbiol. Biotechnol., 76, 117-122.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.26 mutants expressed in Pichia pastoris Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.26 E272K activity optimum at pH 3.2, shows improvement in substrate affinity Aspergillus niger
3.1.3.26 E272Q pH optimum similar to the wild type enzyme Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.26 0.09
-
 myo-inositol hexakisphosphate mutant enzyme E272K, in 0.2 M glycine-HCl, at pH 3.2 Aspergillus niger
3.1.3.26 0.13
-
 myo-inositol hexakisphosphate mutant enzyme E272K, in 0.2 M glycine-HCl, at pH 2.5 Aspergillus niger
3.1.3.26 0.54
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M glycine-HCl, at pH 3.2 Aspergillus niger
3.1.3.26 0.77
-
 myo-inositol hexakisphosphate mutant enzyme E272Q, in 0.2 M glycine-HCl, at pH 3.2 Aspergillus niger
3.1.3.26 4.72
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M glycine-HCl, at pH 2.5 Aspergillus niger
3.1.3.26 4.79
-
 myo-inositol hexakisphosphate mutant enzyme E272Q, in 0.2 M glycine-HCl, at pH 2.5 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.26 75850
-
 2 * 75850, gel filtration Aspergillus niger
3.1.3.26 151700
-
 gel filtration Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.26 Aspergillus niger
-
-
-
3.1.3.26 Aspergillus niger NRRL 3135
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.26 glycoprotein
-
 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Aspergillus niger myo-inositol pentakisphosphate + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Aspergillus niger NRRL 3135 myo-inositol pentakisphosphate + phosphate
-
 ?
3.1.3.26 p-nitrophenyl phosphate + H2O little activity for p-nitrophenyl phosphate above pH 4.0 Aspergillus niger p-nitrophenol + phosphate
-
 ?
3.1.3.26 p-nitrophenyl phosphate + H2O little activity for p-nitrophenyl phosphate above pH 4.0 Aspergillus niger NRRL 3135 p-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.26 dimer 2 * 75850, gel filtration Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.1.3.26 PhyB
-
 Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.26 0.63
-
 myo-inositol hexakisphosphate mutant enzyme E272Q, in 0.2 M glycine-HCl, at pH 2.5 Aspergillus niger
3.1.3.26 3.17
-
 myo-inositol hexakisphosphate mutant enzyme E272K, in 0.2 M glycine-HCl, at pH 2.5 Aspergillus niger
3.1.3.26 3.5
-
 myo-inositol hexakisphosphate mutant enzyme E272K, in 0.2 M glycine-HCl, at pH 3.2 Aspergillus niger
3.1.3.26 9.3
-
 myo-inositol hexakisphosphate mutant enzyme E272Q, in 0.2 M glycine-HCl, at pH 3.2 Aspergillus niger
3.1.3.26 17
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M glycine-HCl, at pH 3.2 Aspergillus niger
3.1.3.26 23.8
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M glycine-HCl, at pH 2.5 Aspergillus niger
3.1.3.26 40.67
-
 myo-inositol hexakisphosphate mutant enzyme E272Q, in 0.2 M glycine-HCl, at pH 2.5 Aspergillus niger
3.1.3.26 88.83
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M glycine-HCl, at pH 2.5 Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.26 2.5
-
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.26 3 3.5 has little activity at pH 3.0-3.5 Aspergillus niger