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Literature summary extracted from

  • Lin, W.; Chan, M.; Goh, L.L.; Sim, T.S.
    Molecular basis for thermal properties of Streptomyces thermovulgaris fumarase C hinge at hydrophilic amino acids R163, E170 and S347 (2007), Appl. Microbiol. Biotechnol., 75, 329-335.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.1.2 synthesis stFUMC is a highly efficient, thermostable fumarase C with industrial potential Streptomyces thermovulgaris

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.2 expression in Escherichia coli BL21 Streptomyces coelicolor
4.2.1.2 expression in Escherichia coli BL21 Streptomyces thermovulgaris

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.2 A347S mutation results in an increase in optimum temperature of 10°C and a fourfold enhancement in specific activity Streptomyces coelicolor
4.2.1.2 G163R mutation results in an increase in optimum temperature of 5°C Streptomyces coelicolor
4.2.1.2 G163R/G170E mutant is more thermostable than wild-type scFUMC. Mutation results in an increase in optimum temperature of 5°C Streptomyces coelicolor
4.2.1.2 G163R/G170E/A347S mutant is more thermostable than wild-type scFUMC. Mutation results in an increase in optimum temperature of 5°C Streptomyces coelicolor
4.2.1.2 G170E mutation results in an increase in optimum temperature of 5°C Streptomyces coelicolor
4.2.1.2 G170E/A347S mutation results in an increase in optimum temperature of 5°C Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 Streptomyces coelicolor
-
-
-
4.2.1.2 Streptomyces thermovulgaris A5Y6J1
-
-

Synonyms

EC Number Synonyms Comment Organism
4.2.1.2 fumarase C
-
Streptomyces coelicolor
4.2.1.2 fumarase C
-
Streptomyces thermovulgaris
4.2.1.2 scFUMC
-
Streptomyces coelicolor
4.2.1.2 stFUMC
-
Streptomyces thermovulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.2 30
-
-
Streptomyces coelicolor
4.2.1.2 50
-
-
Streptomyces thermovulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.2 40
-
24 h, stable Streptomyces thermovulgaris
4.2.1.2 50
-
rapid denaturation at Streptomyces coelicolor