EC Number | Application | Comment | Organism |
---|---|---|---|
4.2.1.2 | synthesis | stFUMC is a highly efficient, thermostable fumarase C with industrial potential | Streptomyces thermovulgaris |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.2 | expression in Escherichia coli BL21 | Streptomyces coelicolor |
4.2.1.2 | expression in Escherichia coli BL21 | Streptomyces thermovulgaris |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.2 | A347S | mutation results in an increase in optimum temperature of 10°C and a fourfold enhancement in specific activity | Streptomyces coelicolor |
4.2.1.2 | G163R | mutation results in an increase in optimum temperature of 5°C | Streptomyces coelicolor |
4.2.1.2 | G163R/G170E | mutant is more thermostable than wild-type scFUMC. Mutation results in an increase in optimum temperature of 5°C | Streptomyces coelicolor |
4.2.1.2 | G163R/G170E/A347S | mutant is more thermostable than wild-type scFUMC. Mutation results in an increase in optimum temperature of 5°C | Streptomyces coelicolor |
4.2.1.2 | G170E | mutation results in an increase in optimum temperature of 5°C | Streptomyces coelicolor |
4.2.1.2 | G170E/A347S | mutation results in an increase in optimum temperature of 5°C | Streptomyces coelicolor |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.2 | Streptomyces coelicolor | - |
- |
- |
4.2.1.2 | Streptomyces thermovulgaris | A5Y6J1 | - |
- |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.2 | fumarase C | - |
Streptomyces coelicolor |
4.2.1.2 | fumarase C | - |
Streptomyces thermovulgaris |
4.2.1.2 | scFUMC | - |
Streptomyces coelicolor |
4.2.1.2 | stFUMC | - |
Streptomyces thermovulgaris |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.2 | 30 | - |
- |
Streptomyces coelicolor |
4.2.1.2 | 50 | - |
- |
Streptomyces thermovulgaris |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.2 | 40 | - |
24 h, stable | Streptomyces thermovulgaris |
4.2.1.2 | 50 | - |
rapid denaturation at | Streptomyces coelicolor |