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Literature summary extracted from

  • Byun, S.G.; Kim, M.D.; Lee, W.H.; Lee, K.J.; Han, N.S.; Seo, J.H.
    Production of GDP-L-fucose, L-fucose donor for fucosyloligosaccharide synthesis, in recombinant Escherichia coli (2007), Appl. Microbiol. Biotechnol., 74, 768-775.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.271 isopropyl-beta-D-thioglucopyranoside
-
 Escherichia coli K-12

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.271 gene wcaG, overexpression in strain BL21(DE3) together with GDP-D-mannose-4,6-dehydratase Escherichia coli K-12
4.2.1.47 GDP-D-mannose-4,6-dehydratase and GDP-4-keto-6-deoxymannose 3, 5-epimerase 4-reductase, the two crucial enzymes for the de novo GDP-L-fucose biosynthesis, are overexpressed in recombinant Escherichia coli by constructing inducible overexpression vectors. Optimum expression conditions in recombinant Escherichia coli BL21(DE3) are 25°C and 0.1 mM isopropyl-beta-D-thioglucopyranoside. Maximum GDP-L-fucose concentration of 38.9 mg/l is obtained in a glucose-limited fed-batch cultivation, and it is enhanced further by coexpression of NADPH-regenerating glucose-6-phosphate dehydrogenase encoded by the zwf gene to achieve 55.2 mg/l GDP-L-fucose under the same cultivation condition Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.271 additional information effective GDP-L-fucose production in recombinant Escherichia coli by expression of GDP-D-mannose-4,6-dehydratase and GDP-4-keto-6-deoxymannose 3,5-epimerase 4-reductase, method optimization at 25°C and 0.1 mM isopropyl-beta-D-thioglucopyranoside, overview Escherichia coli K-12

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.271 GDP-4-dehydro-6-deoxy-D-mannose + NADPH Escherichia coli K-12 the enzyme is crucial in the biosynthesis of GDP-L-fucose together with GDP-D-mannose-4,6-dehydratase, overview GDP-L-fucose + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.271 Escherichia coli K-12
-
 gene wcaG
-
4.2.1.47 Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.271 additional information
-
-
 Escherichia coli K-12

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.271 GDP-4-dehydro-6-deoxy-D-mannose + NADPH
-
 Escherichia coli K-12 GDP-L-fucose + NADP+
-
 r
1.1.1.271 GDP-4-dehydro-6-deoxy-D-mannose + NADPH the enzyme is crucial in the biosynthesis of GDP-L-fucose together with GDP-D-mannose-4,6-dehydratase, overview Escherichia coli K-12 GDP-L-fucose + NADP+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.271 GDP-4-keto-6-deoxymannose 3, 5-epimerase 4-reductase
-
 Escherichia coli K-12
1.1.1.271 wcaG
-
 Escherichia coli K-12

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.271 NADP+
-
 Escherichia coli K-12
1.1.1.271 NADPH dependent on Escherichia coli K-12