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Literature summary extracted from

  • Durban, M.A.; Silbersack, J.; Schweder, T.; Schauer, F.; Bornscheuer, U.T.
    High level expression of a recombinant phospholipase C from Bacillus cereus in Bacillus subtilis (2007), Appl. Microbiol. Biotechnol., 74, 634-639.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.3 expression in Bacillus subtilis Bacillus cereus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.3 additional information amino acid sequence differs from that of strain SBUG516 by substitution D173E Bacillus cereus
3.1.4.3 additional information amino acid sequence differs from that of strain SBUG516 by substitution E173D Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Bacillus cereus
-
-
-
3.1.4.3 Bacillus cereus SBUG318
-
-
-
3.1.4.3 Bacillus cereus SBUG516
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.3
-
 Bacillus cereus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.3 13190
-
 37°C Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 p-nitrophenylphosphorylcholine + H2O
-
 Bacillus cereus p-nitrophenol + phosphorylcholine
-
 ?
3.1.4.3 p-nitrophenylphosphorylcholine + H2O
-
 Bacillus cereus SBUG516 p-nitrophenol + phosphorylcholine
-
 ?
3.1.4.3 p-nitrophenylphosphorylcholine + H2O
-
 Bacillus cereus SBUG318 p-nitrophenol + phosphorylcholine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.3 60
-
-
 Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.3 5
-
-
 Bacillus cereus