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Literature summary extracted from

  • Kakugawa, S.; Fushinobu, S.; Wakagi, T.; Shoun, H.
    Characterization of a thermostable carboxylesterase from the hyperthermophilic bacterium Thermotoga maritima (2007), Appl. Microbiol. Biotechnol., 74, 585-591.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 expression in Escherichia coli, with c-terminal His-tag and wizthout the N-terminal hydrophobic region Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 2-mercaptoethanol 94% residual activity Thermotoga maritima
3.1.1.1 diethyl dicarbonate
-
 Thermotoga maritima
3.1.1.1 EDTA 81% residual activity Thermotoga maritima
3.1.1.1 EGTA 39% residual activity Thermotoga maritima
3.1.1.1 Mn2+ 5 mM, 62% of initial activity Thermotoga maritima
3.1.1.1 Ni2+ 5 mM, 67% of initial activity Thermotoga maritima
3.1.1.1 O,O'-bis(2-aminoethyl)ethylenglycol-N,N,N',N'-tetraacetic acid
-
 Thermotoga maritima
3.1.1.1 Phenylmethyl sulfonylfluoride 11% residual activity Thermotoga maritima
3.1.1.1 phenylmethylsulfonyl fluoride
-
 Thermotoga maritima
3.1.1.1 Zn2+ 5 mM, 41% of initial activity Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.00031
-
 p-nitrophenyl decanoate 60°C, pH 7.5 Thermotoga maritima
3.1.1.1 0.0021
-
 p-nitrophenyl myristate 60°C, pH 7.5 Thermotoga maritima
3.1.1.1 0.0047
-
 p-nitrophenyl caprylate 60°C, pH 7.5 Thermotoga maritima
3.1.1.1 0.243
-
 p-nitrophenyl butanoate 60°C, pH 7.5 Thermotoga maritima
3.1.1.1 1.15
-
 p-nitrophenyl acetate 60°C, pH 7.5 Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.1 Ca2+ 5 mM, 236% of initial activity Thermotoga maritima
3.1.1.1 Fe2+ 5 mM, 196% of initial activity Thermotoga maritima
3.1.1.1 K+ 5 mM, 137% of initial activity Thermotoga maritima
3.1.1.1 Mg2+ 5 mM, 214% of initial activity Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 39313
-
 x * 40000, SDS-PAGE, x * 39313, calculated, His-tagged protein Thermotoga maritima
3.1.1.1 40000
-
 x * 40000, SDS-PAGE, x * 39313, calculated, His-tagged protein Thermotoga maritima

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.1 dimethyl sulfoxide 5%, 146% of initial activity Thermotoga maritima
3.1.1.1 dimethylformamide 5%, 99% of initial activity Thermotoga maritima
3.1.1.1 dioxane 5%, 141% of initial activity Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 recombinant protein Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl decanoate + H2O
-
 Thermotoga maritima 4-nitrophenol + decanoate
-
 ?
3.1.1.1 p-nitrophenyl acetate + H2O
-
 Thermotoga maritima p-nitrophenol + acetate
-
 ?
3.1.1.1 p-nitrophenyl butanoate + H2O
-
 Thermotoga maritima p-nitrophenol + butanoate
-
 ?
3.1.1.1 p-nitrophenyl caprylate + H2O
-
 Thermotoga maritima p-nitrophenol + caprylate
-
 ?
3.1.1.1 p-nitrophenyl decanoate
-
 Thermotoga maritima p-nitrophenol + octanoate
-
 ?
3.1.1.1 p-nitrophenyl myristate + H2O
-
 Thermotoga maritima p-nitrophenol + myristate
-
 ?
3.1.1.1 p-nitrophenyl palmitate + H2O 50% of the activity with p-nitrophenyl decanoate Thermotoga maritima p-nitrophenol + palmitate
-
 ?
3.1.1.1 p-nitrophenyl stearate + H2O 50% of the activity with p-nitrophenyl decanoate Thermotoga maritima p-nitrophenol + stearate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 ? x * 40000, SDS-PAGE, x * 39313, calculated, His-tagged protein Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 60
-
-
 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 60
-
 90 min, stable Thermotoga maritima
3.1.1.1 80
-
 30 min, 50% residual activity Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.1 4.9
-
 p-nitrophenyl myristate 60°C, pH 7.5 Thermotoga maritima
3.1.1.1 10.8
-
 p-nitrophenyl decanoate 60°C, pH 7.5 Thermotoga maritima
3.1.1.1 13
-
 p-nitrophenyl caprylate 60°C, pH 7.5 Thermotoga maritima
3.1.1.1 14
-
 p-nitrophenyl butanoate 60°C, pH 7.5 Thermotoga maritima
3.1.1.1 50
-
 p-nitrophenyl acetate 60°C, pH 7.5 Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 6.5 7
-
 Thermotoga maritima