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Literature summary extracted from

  • Teng, D.; Fan, Y.; Yang, Y.L.; Tian, Z.G.; Luo, J.; Wang, J.H.
    Codon optimization of Bacillus licheniformis beta-1,3-1,4-glucanase gene and its expression in Pichia pastoris (2007), Appl. Microbiol. Biotechnol., 74, 1074-1083.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.6 2-mercaptoethanol 0.1%, activates Bacillus licheniformis
3.2.1.73 beta-mercaptoethanol 0.1% Bacillus licheniformis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.6 expression in Pichia pastoris. The beta-1,3-1,4-glucanase gene is modified and expressed according to its preferred codon usage Bacillus licheniformis
3.2.1.73 expressed in Pichia pastoris strain GS115 Bacillus licheniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.6 Cu2+ 10 mM Bacillus licheniformis
3.2.1.6 Zn2+ 10 mM Bacillus licheniformis
3.2.1.73 Cu2+ 10 mM Bacillus licheniformis
3.2.1.73 additional information no significant effects are found for Ca2+, Mg2+, glycerol, D-sorbitol, boric acid, and EDTA Bacillus licheniformis
3.2.1.73 Zn2+ 10 mM Bacillus licheniformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.6 Co2+ activates Bacillus licheniformis
3.2.1.6 Fe2+ activates Bacillus licheniformis
3.2.1.6 Mn2+ activates Bacillus licheniformis
3.2.1.73 Co2+ activates at 10 mM Bacillus licheniformis
3.2.1.73 Fe2+ activates at 10 mM Bacillus licheniformis
3.2.1.73 Mn2+ activates at 10 mM Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.6 34000
-
 x * 34000, SDS-PAGE Bacillus licheniformis
3.2.1.73 34000
-
 SDS-PAGE Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.6 Bacillus licheniformis Q8GMY0
-
-
3.2.1.73 Bacillus licheniformis
-
-
-
3.2.1.73 Bacillus licheniformis EGW039 / CGMCC 0635
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.6 glycoprotein
-
 Bacillus licheniformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 1280
-
 recombinant enzyme, using barley beta-glucan as a substrate, at 50°C and pH 6.0 Bacillus licheniformis
3.2.1.73 1335
-
 recombinant enzyme, using lichenan as a substrate, at 50°C and pH 6.0 Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.6 beta-glucan + H2O
-
 Bacillus licheniformis ?
-
 ?
3.2.1.6 lichenan + H2O
-
 Bacillus licheniformis ?
-
 ?
3.2.1.6 additional information no activity on cellulose Bacillus licheniformis ?
-
 ?
3.2.1.73 barley beta-glucan + H2O
-
 Bacillus licheniformis ?
-
 ?
3.2.1.73 barley beta-glucan + H2O
-
 Bacillus licheniformis EGW039 / CGMCC 0635 ?
-
 ?
3.2.1.73 lichenan + H2O
-
 Bacillus licheniformis ?
-
 ?
3.2.1.73 lichenan + H2O
-
 Bacillus licheniformis EGW039 / CGMCC 0635 ?
-
 ?
3.2.1.73 additional information no activity on cellulose Bacillus licheniformis ?
-
 ?
3.2.1.73 additional information no activity on cellulose Bacillus licheniformis EGW039 / CGMCC 0635 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.6 ? x * 34000, SDS-PAGE Bacillus licheniformis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.6 beta-1,3-1,4-glucanase
-
 Bacillus licheniformis
3.2.1.73 beta-1,3-1,4-glucanase
-
 Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.6 45
-
 recombinant enzyme Bacillus licheniformis
3.2.1.73 45
-
 recombinant enzyme Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.6 50
-
 30 min, 5% loss of activity Bacillus licheniformis
3.2.1.6 60
-
 30 min, 40% loss of activity Bacillus licheniformis
3.2.1.6 70
-
 30 min, complete loss of activity Bacillus licheniformis
3.2.1.73 50 70 after incubation at 50, 60, and 70°C for 30 min, the residual activity is 95, 60, and 0%, respectively Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.6 6
-
 recombinant enzyme Bacillus licheniformis
3.2.1.73 6
-
 recombinant enzyme Bacillus licheniformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.6 4.5 8 pH 4.5: abput 40% of maximal activity, pH 8.0: about 55% of maximal activity Bacillus licheniformis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.6 3
-
 12 h, enzyme retains 80% of its original activity Bacillus licheniformis
3.2.1.6 4 6 stable Bacillus licheniformis
3.2.1.6 8
-
 12 h, enzyme retains 80% of its original activity Bacillus licheniformis
3.2.1.73 3
-
 retains approximately 80% of original activity after treatment at pH 3.0 for 12 h Bacillus licheniformis
3.2.1.73 4 6 the recombinant enzyme is stable between pH 4.0 and 6.0, having 85% of the original activity Bacillus licheniformis
3.2.1.73 8
-
 retains approximately 80% of original activity after treatment at pH 8.0 for 12 h Bacillus licheniformis