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Literature summary extracted from

  • Cho, K.M.; Hong, S.Y.; Lee, S.M.; Kim, Y.H.; Kahng, G.G.; Kim, H.; Yun, H.D.
    A cel44C-man26A gene of endophytic Paenibacillus polymyxa GS01 has multi-glycosyl hydrolases in two catalytic domains (2006), Appl. Microbiol. Biotechnol., 73, 618-630.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expression in Escherichia coli Paenibacillus polymyxa

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.73 D100A 36% relative activity compared to the wild type enzyme Paenibacillus polymyxa
3.2.1.73 D106A 34% relative activity compared to the wild type enzyme Paenibacillus polymyxa
3.2.1.73 D195A 36% relative activity compared to the wild type enzyme Paenibacillus polymyxa
3.2.1.73 D220A 32% relative activity compared to the wild type enzyme Paenibacillus polymyxa
3.2.1.73 D258A 35% relative activity compared to the wild type enzyme Paenibacillus polymyxa
3.2.1.73 D314A 99% relative activity compared to the wild type enzyme Paenibacillus polymyxa
3.2.1.73 D317A 31% relative activity compared to the wild type enzyme Paenibacillus polymyxa
3.2.1.73 E196A 39% relative activity compared to the wild type enzyme Paenibacillus polymyxa
3.2.1.73 E222A no activity Paenibacillus polymyxa
3.2.1.73 E242A 32% relative activity compared to the wild type enzyme Paenibacillus polymyxa
3.2.1.73 E262A 36% relative activity compared to the wild type enzyme Paenibacillus polymyxa
3.2.1.73 E323A 32% relative activity compared to the wild type enzyme Paenibacillus polymyxa
3.2.1.73 E91A no activity Paenibacillus polymyxa
3.2.1.78 E222A no catalytic acitivity Paenibacillus polymyxa
3.2.1.78 E91A no catalytic acitivity Paenibacillus polymyxa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 145294
-
 x * 145294, calculated Paenibacillus polymyxa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Paenibacillus polymyxa
-
-
-
3.2.1.73 Paenibacillus polymyxa GS01
-
-
-
3.2.1.78 Paenibacillus polymyxa
-
 enzyme belongs to glycosyl hydrolase family 26. Protein additionally contains a glycosyl hydroxylase family 44 catalytic domain, a fibronectin domain type 3, and a cellulose-binding module type 3
-
3.2.1.78 Paenibacillus polymyxa GS01
-
 enzyme belongs to glycosyl hydrolase family 26. Protein additionally contains a glycosyl hydroxylase family 44 catalytic domain, a fibronectin domain type 3, and a cellulose-binding module type 3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 recombinant protein Paenibacillus polymyxa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 234
-
 mutant enzyme D317A, at 50°C Paenibacillus polymyxa
3.2.1.73 240
-
 mutant enzyme D220A, at 50°C Paenibacillus polymyxa
3.2.1.73 241
-
 mutant enzyme E323A, at 50°C Paenibacillus polymyxa
3.2.1.73 245
-
 mutant enzyme E242A, at 50°C Paenibacillus polymyxa
3.2.1.73 258
-
 mutant enzyme D106A, at 50°C Paenibacillus polymyxa
3.2.1.73 266
-
 mutant enzyme D258A, at 50°C Paenibacillus polymyxa
3.2.1.73 271
-
 mutant enzyme E262A, at 50°C Paenibacillus polymyxa
3.2.1.73 273
-
 mutant enzyme D100A, at 50°C Paenibacillus polymyxa
3.2.1.73 274
-
 mutant enzyme D195A, at 50°C Paenibacillus polymyxa
3.2.1.73 296
-
 mutant enzyme D196A, at 50°C Paenibacillus polymyxa
3.2.1.73 748
-
 mutant enzyme D314A, at 50°C Paenibacillus polymyxa
3.2.1.73 754
-
 wild type enzyme, at 50°C Paenibacillus polymyxa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 lichenan + H2O
-
 Paenibacillus polymyxa ?
-
 ?
3.2.1.73 lichenan + H2O
-
 Paenibacillus polymyxa GS01 ?
-
 ?
3.2.1.78 locust bean gum + H2O
-
 Paenibacillus polymyxa ?
-
 ?
3.2.1.78 locust bean gum + H2O
-
 Paenibacillus polymyxa GS01 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 145294, calculated Paenibacillus polymyxa

Synonyms

EC Number Synonyms Comment Organism
3.2.1.73 Lichenase the multifunctional enzyme domain GH44 possesses cellulase, xylanase, and lichenase activities Paenibacillus polymyxa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 50
-
-
 Paenibacillus polymyxa
3.2.1.78 50
-
-
 Paenibacillus polymyxa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 7
-
-
 Paenibacillus polymyxa
3.2.1.78 5
-
 xylanase and mannanase activities of enzyme Paenibacillus polymyxa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.78 Paenibacillus polymyxa calculated
-
 5.5