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Literature summary extracted from

  • Shin, H.D.; Chen, R.R.
    A type B feruloyl esterase from Aspergillus nidulans with broad pH applicability (2007), Appl. Microbiol. Biotechnol., 73, 1323-1330.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 successfully cloned from the genomic DNA and expressed in Saccharomyces cerevisiae Aspergillus nidulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.248
-
 methyl ferulate 40°C, pH 7.0 Aspergillus nidulans
3.1.1.73 0.387
-
 methyl caffeate 40°C, pH 7.0 Aspergillus nidulans
3.1.1.73 0.659
-
 methyl p-coumarate 40°C, pH 7.0 Aspergillus nidulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 56000
-
 x * 56000, deglycosylated enzyme, SDS-PAGE Aspergillus nidulans
3.1.1.73 56107
-
 x * 56107, calculated from sequence Aspergillus nidulans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus nidulans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73
-
 Aspergillus nidulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 14.9
-
-
 Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 methyl caffeate + H2O
-
 Aspergillus nidulans caffeic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Aspergillus nidulans ferulic acid + methanol
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 Aspergillus nidulans p-coumaric acid + methanol
-
 ?
3.1.1.73 additional information no activity with methyl sinapate Aspergillus nidulans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 56000, deglycosylated enzyme, SDS-PAGE Aspergillus nidulans
3.1.1.73 ? x * 56107, calculated from sequence Aspergillus nidulans

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 AN1772.2
-
 Aspergillus nidulans
3.1.1.73 type B FAE
-
 Aspergillus nidulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 45
-
-
 Aspergillus nidulans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73 30 50 30°C: about 80% of maximal activity, 50°C: about 30% of maximal activity Aspergillus nidulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 45
-
 stable up to Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 7
-
-
 Aspergillus nidulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 5 8.5 pH 5.0: about 50% of maximal activity, pH 8.5: about 40% of maximal activity Aspergillus nidulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.73 4
-
 4°C, 12 h, 15% loss of activity Aspergillus nidulans
3.1.1.73 8
-
 4°C, 12 h, no loss of activity Aspergillus nidulans
3.1.1.73 10
-
 4°C, 12 h, about 20% loss of activity Aspergillus nidulans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.73 Aspergillus nidulans calculated from sequence
-
 4.7