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Literature summary extracted from

  • Bhatti, H.N.; Rashid, M.H.; Nawaz, R.; Khalid, A.M.; Asgher, M.; Jabbar, A.
    Effect of aniline coupling on kinetic and thermodynamic properties of Fusarium solani glucoamylase (2007), Appl. Microbiol. Biotechnol., 73, 1290-1298.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.3 additional information purified glucoamylase is chemically modified by cross-linking with aniline hydrochloride in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide for 1, 7, or 13 min, resulting in aniline-coupled glucoamylase-1/ACG-1, aniline-coupled glucoamylase-7/ACG-7, and 13 min aniline-coupled glucoamylase-13/ACG-13, the aniline coupling of GA has profound enhancing effects on temperature, pH optima, and pKa’s of active site residues, overview Fusarium solani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 additional information kinetics of native enzyme, and aniline-coupled glucoamylase-1/ACG-1, aniline-coupled glucoamylase-7/ACG-7, and 13 min aniline-coupled glucoamylase-13/ACG-13, overview Fusarium solani
3.2.1.3 1.4
-
 starch pH 4.5, 40°C, ACG-7 Fusarium solani
3.2.1.3 1.6
-
 starch pH 4.5, 40°C, native enzyme Fusarium solani
3.2.1.3 1.7
-
 starch pH 4.5, 40°C, ACG-13 Fusarium solani
3.2.1.3 2
-
 starch pH 4.5, 40°C, ACG-1 Fusarium solani

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Fusarium solani
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 additional information
-
 activities of native enzyme, and aniline-coupled glucoamylase-1/ACG-1, aniline-coupled glucoamylase-7/ACG-7, and 13 min aniline-coupled glucoamylase-13/ACG-13, overview Fusarium solani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 starch + H2O
-
 Fusarium solani ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 glucoamylase
-
 Fusarium solani

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 40
-
 assay at, native enzyme Fusarium solani
3.2.1.3 55
-
 assay at, aniline-coupled glucoamylases Fusarium solani

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 50
-
 native enzyme shows a half-life of 128.37 min Fusarium solani
3.2.1.3 60
-
 native enzyme shows a half-life of 25.67 min, ACG-13 shows a half-life of 67.3 min Fusarium solani

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.3 217
-
 starch pH 4.5, 40°C, native enzyme Fusarium solani
3.2.1.3 354
-
 starch pH 4.5, 40°C, ACG-13 Fusarium solani
3.2.1.3 367
-
 starch pH 4.5, 40°C, ACG-7 Fusarium solani
3.2.1.3 487
-
 starch pH 4.5, 40°C, ACG-1 Fusarium solani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4.5
-
 native enzyme and aniline-coupled glucoamylase ACG-1 Fusarium solani
3.2.1.3 5.5
-
 aniline-coupled glucoamylases ACG-7 and ACG-13 Fusarium solani