Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Teng, D.; Wang, J.H.; Fan, Y.; Yang, Y.L.; Tian, Z.G.; Luo, J.; Yang, G.P.; Zhang, F.
    Cloning of beta-1,3-1,4-glucanase gene from Bacillus licheniformis EGW039 (CGMCC 0635) and its expression in Escherichia coli BL21 (DE3) (2006), Appl. Microbiol. Biotechnol., 72, 705-712.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.6 expression in Escherichia coli BL21 (DE3) Bacillus licheniformis
3.2.1.73 expressed in Escherichia coli strain BL21 (DE3) Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.6 28000
-
 x * 28000, recombinant enzyme, SDS-PAGE Bacillus licheniformis
3.2.1.73 28000
-
 recombinant enzyme with a six-His tag at the N terminus, SDS-PAGE Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.6 Bacillus licheniformis
-
-
-
3.2.1.6 Bacillus licheniformis EGW039 / CGMCC 0635
-
-
-
3.2.1.73 Bacillus licheniformis
-
-
-
3.2.1.73 Bacillus licheniformis EGW039 / CGMCC 0635
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.6 recombinant enzyme Bacillus licheniformis
3.2.1.73 Ni-NTA His Bind resin column chromatography Bacillus licheniformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.6 2479
-
-
 Bacillus licheniformis
3.2.1.73 283.8
-
 recombinant enzyme from crude extract, using barley beta-glucan as a substrate Bacillus licheniformis
3.2.1.73 1906
-
 recombinant enzyme, using lichenan as a substrate Bacillus licheniformis
3.2.1.73 2479
-
 recombinant enzyme after 8.74fold purification, using barley beta-glucan as a substrate Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.6 beta-glucan + H2O barley beta-glucan Bacillus licheniformis ?
-
 ?
3.2.1.6 beta-glucan + H2O barley beta-glucan Bacillus licheniformis EGW039 / CGMCC 0635 ?
-
 ?
3.2.1.6 lichenan + H2O
-
 Bacillus licheniformis ?
-
 ?
3.2.1.6 lichenan + H2O
-
 Bacillus licheniformis EGW039 / CGMCC 0635 ?
-
 ?
3.2.1.73 barley beta-glucan + H2O
-
 Bacillus licheniformis ?
-
 ?
3.2.1.73 barley beta-glucan + H2O
-
 Bacillus licheniformis EGW039 / CGMCC 0635 ?
-
 ?
3.2.1.73 lichenan + H2O
-
 Bacillus licheniformis ?
-
 ?
3.2.1.73 lichenan + H2O
-
 Bacillus licheniformis EGW039 / CGMCC 0635 ?
-
 ?
3.2.1.73 additional information activity against 1% carboxymethylcellulose is not observed Bacillus licheniformis ?
-
 ?
3.2.1.73 additional information activity against 1% carboxymethylcellulose is not observed Bacillus licheniformis EGW039 / CGMCC 0635 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.6 ? x * 28000, recombinant enzyme, SDS-PAGE Bacillus licheniformis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.6 beta-1,3-1,4-glucanase
-
 Bacillus licheniformis
3.2.1.73 beta-1,3-1,4-glucanase
-
 Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.6 40
-
 recombinant enzyme Bacillus licheniformis
3.2.1.73 40
-
 recombinant enzyme Bacillus licheniformis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.6 30 70 30°C: about 90% of maximal activity, 70°C: about 50% of maximal activity Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.6 5.6
-
 recombinant enzyme Bacillus licheniformis
3.2.1.73 5.6
-
 recombinant enzyme Bacillus licheniformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.6 4.5 8 pH 4.5: about 60% of maximal activity, pH 8: about 50% of maximal activity Bacillus licheniformis