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Literature summary extracted from

  • Ike, M.; Isami, K.; Tanabe, Y.; Nogawa, M.; Ogasawara, W.; Okada, H.; Morikawa, Y.
    Cloning and heterologous expression of the exo-beta-D-glucosaminidase-encoding gene (gls93) from a filamentous fungus, Trichoderma reesei PC-3-7 (2006), Appl. Microbiol. Biotechnol., 72, 687-695.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.165 expression in Pichia pastoris Trichoderma reesei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.165 100000
-
 x * 100000, the recombinant Gls93 has the two molecular forms, ca. 105000 Da and 100000 Da, whose difference is caused by N-glycosylation, SDS-PAGE Trichoderma reesei
3.2.1.165 105000
-
 x * 105000, the recombinant Gls93 has the two molecular forms, ca. 105000 Da and 100000 Da, whose difference is caused by N-glycosylation, SDS-PAGE Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.165 Trichoderma reesei Q4R1C4 precursor
-
3.2.1.165 Trichoderma reesei PC-3-7 Q4R1C4 precursor
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.165 glycoprotein the recombinant Gls93 has the two molecular forms, ca. 105000 Da and 100000 Da, whose difference is caused by N-glycosylation Trichoderma reesei

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.165 recombinant enzyme Trichoderma reesei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.165 culture filtrate of cells grown on N-acetyl-D-glucosamine Trichoderma reesei
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.165 chitopentaose + H2O cleaved to chitotetraose and glucosamine in the initial stage of the reaction Trichoderma reesei ?
-
 ?
3.2.1.165 chitopentaose + H2O cleaved to chitotetraose and glucosamine in the initial stage of the reaction Trichoderma reesei PC-3-7 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.165 ? x * 100000, the recombinant Gls93 has the two molecular forms, ca. 105000 Da and 100000 Da, whose difference is caused by N-glycosylation, SDS-PAGE Trichoderma reesei
3.2.1.165 ? x * 105000, the recombinant Gls93 has the two molecular forms, ca. 105000 Da and 100000 Da, whose difference is caused by N-glycosylation, SDS-PAGE Trichoderma reesei

Synonyms

EC Number Synonyms Comment Organism
3.2.1.165 exo-beta-D-glucosaminidase
-
 Trichoderma reesei
3.2.1.165 Gls93
-
 Trichoderma reesei