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Literature summary extracted from

  • Kato, Y.; Asano, Y.
    Molecular and enzymatic analysis of the "aldoxime-nitrile pathway" in the glutaronitrile degrader Pseudomonas sp. K-9 (2006), Appl. Microbiol. Biotechnol., 70, 92-101.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.8.1.2 overexpression in Escherichia coli as His tagged fusion protein at the N-terminus, and as tagged fusion protein at the C-terminus Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.8.1.2 KCN
-
 Pseudomonas sp.
4.8.1.2 NH2OH
-
 Pseudomonas sp.
4.8.1.2 phenylhydrazine
-
 Pseudomonas sp.
4.8.1.2 Z-phenylacetaldoxime
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.8.1.2 0.538
-
 (E/Z)-isobutyraldoxime
-
 Pseudomonas sp.
4.8.1.2 0.778
-
 (E/Z)-propionaldoxime
-
 Pseudomonas sp.
4.8.1.2 0.882
-
 (E/Z)-4-phenybutyraldoxime
-
 Pseudomonas sp.
4.8.1.2 0.975
-
 (Z)-3-phenylpropionaldoxime
-
 Pseudomonas sp.
4.8.1.2 0.991
-
 (Z)-phenylacetaldoxime
-
 Pseudomonas sp.
4.8.1.2 1.33
-
 (E/Z)-isovaleraldoxime
-
 Pseudomonas sp.
4.8.1.2 2.16
-
 (E/Z)-n-butyraldoxime
-
 Pseudomonas sp.
4.8.1.2 3.12
-
 (E/Z)-n-capronaldoxime
-
 Pseudomonas sp.
4.8.1.2 3.78
-
 (E/Z)-n-valeraldoxime
-
 Pseudomonas sp.
4.8.1.2 4.07
-
 (E/Z)-2-phenylpropionaldoxime
-
 Pseudomonas sp.
4.8.1.2 5.96
-
 (E/Z)-cyclohexanecarboxaldehydeoxime
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.8.1.2 Ag+ inhibitory Pseudomonas sp.
4.8.1.2 Co2+ inhibitory Pseudomonas sp.
4.8.1.2 Cr3+ inhibitory Pseudomonas sp.
4.8.1.2 Cu+ inhibitory Pseudomonas sp.
4.8.1.2 Fe3+ inhibitory Pseudomonas sp.
4.8.1.2 Pb2+ inhibitory Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.8.1.2 42500
-
 2 * 42500, gel filtration Pseudomonas sp.
4.8.1.2 44510
-
 sequence Pseudomonas sp.
4.8.1.2 85000
-
 SDS-PAGE Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.8.1.2 Pseudomonas sp. Q4W7T3 K-9
-
4.8.1.2 Pseudomonas sp. K-9 Q4W7T3 K-9
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.8.1.2 by using a TALON column Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.8.1.2 (E/Z)-2-phenylpropionaldoxime
-
 Pseudomonas sp. 2-phenylpropionitrile + H2O
-
 ?
4.8.1.2 (E/Z)-2-phenylpropionaldoxime
-
 Pseudomonas sp. K-9 2-phenylpropionitrile + H2O
-
 ?
4.8.1.2 (E/Z)-3-phenylpropionaldoxime
-
 Pseudomonas sp. 3-phenylpropionitrile + H2O
-
 ?
4.8.1.2 (E/Z)-4-phenybutyraldoxime
-
 Pseudomonas sp. 4-phenybutyrnitrile + H2O
-
 ?
4.8.1.2 (E/Z)-cyclohexanecarboxaldehyde oxime
-
 Pseudomonas sp. cyclohexanenitrile + H2O
-
 ?
4.8.1.2 (E/Z)-isobutyraldoxime
-
 Pseudomonas sp. isobutyrnitrile + H2O
-
 ?
4.8.1.2 (E/Z)-isovaleraldoxime
-
 Pseudomonas sp. isovalernitrile + H2O
-
 ?
4.8.1.2 (E/Z)-n-butyraldoxime
-
 Pseudomonas sp. n-butyrnitrile + H2O
-
 ?
4.8.1.2 (E/Z)-n-capronaldoxime
-
 Pseudomonas sp. n-capronnitrile + H2O
-
 ?
4.8.1.2 (E/Z)-n-valeraldoxime
-
 Pseudomonas sp. n-valernitrile + H2O
-
 ?
4.8.1.2 (E/Z)-propionaldoxime
-
 Pseudomonas sp. propionitrile + H2O
-
 ?
4.8.1.2 (E/Z)-propionaldoxime
-
 Pseudomonas sp. K-9 propionitrile + H2O
-
 ?
4.8.1.2 (Z)-3-phenylpropionaldoxime
-
 Pseudomonas sp. 3-phenylpropiononitrile + H2O
-
 ?
4.8.1.2 (Z)-3-phenylpropionaldoxime
-
 Pseudomonas sp. K-9 3-phenylpropiononitrile + H2O
-
 ?
4.8.1.2 (Z)-phenylacetaldoxime
-
 Pseudomonas sp. phenylacetonitrile + H2O
-
 ?
4.8.1.2 (Z)-phenylacetaldoxime
-
 Pseudomonas sp. K-9 phenylacetonitrile + H2O
-
 ?
4.8.1.2 an aliphatic aldoxime
-
 Pseudomonas sp. an aliphatic nitrile + H2O
-
 ?
4.8.1.2 an aliphatic aldoxime
-
 Pseudomonas sp. K-9 an aliphatic nitrile + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.8.1.2 dimer 2 * 42500, gel filtration Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
4.8.1.2 aliphatic aldoxime dehydratase
-
 Pseudomonas sp.
4.8.1.2 OXD
-
 Pseudomonas sp.
4.8.1.2 OxdK
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.8.1.2 20 30
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.8.1.2 30
-
 above 30°C Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8.1.2 7
-
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.8.1.2 5.5 6.5
-
 Pseudomonas sp.