EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.162 | isopropyl-1-thio-beta-D-galactoside | induction of protein expression at a final concentration of 0.5 mM | Bifidobacterium longum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.162 | Escherichia coli strains select96 and BL21(DE3) are used as hosts for cloning and expression. | Bifidobacterium longum |
2.7.1.162 | expression in Escherichia coli | Bifidobacterium longum |
EC Number | General Stability | Organism |
---|---|---|
2.7.1.162 | loses half of enzyme activity during incubation for 30 min at 50°C in 0.1 M Tris buffer (pH 8.5) | Bifidobacterium longum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.162 | 0.065 | - |
N-acetyl-alpha-D-galactosamine | - |
Bifidobacterium longum | |
2.7.1.162 | 0.065 | - |
N-acetyl-alpha-D-galactosamine | pH 8.5, 30°C | Bifidobacterium longum | |
2.7.1.162 | 0.118 | - |
N-acetyl-alpha-D-glucosamine | - |
Bifidobacterium longum | |
2.7.1.162 | 0.118 | - |
N-acetyl-alpha-D-glucosamine | pH 8.5, 30°C | Bifidobacterium longum | |
2.7.1.162 | 0.172 | - |
ATP | cosubstrate: N-acetyl-alpha-D-glucosamine, pH 8.5, 30°C | Bifidobacterium longum | |
2.7.1.162 | 0.172 | - |
ATP | at N-acetyl-alpha-D-glucosamine saturation | Bifidobacterium longum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.162 | Ca2+ | 1 mM, 1% relative activity compared to Mg2+ | Bifidobacterium longum | |
2.7.1.162 | Co2+ | 21% of the activity with Mg2+ | Bifidobacterium longum | |
2.7.1.162 | Co2+ | 1 mM, 21% relative activity compared to Mg2+ | Bifidobacterium longum | |
2.7.1.162 | Mg2+ | requires a divalent cation, with Mg2+ resulting in the greatest stimulation of enzyme activity | Bifidobacterium longum | |
2.7.1.162 | Mg2+ | 1 mM, 100% relative activity | Bifidobacterium longum | |
2.7.1.162 | Mn2+ | 1 mM, 7% relative activity compared to Mg2+ | Bifidobacterium longum | |
2.7.1.162 | Ni2+ | 1 mM, 4% relative activity compared to Mg2+ | Bifidobacterium longum | |
2.7.1.162 | Zn2+ | 1 mM, 8% relative activity compared to Mg2+ | Bifidobacterium longum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.162 | ATP + N-acetyl-alpha-D-galactosamine | Bifidobacterium longum | the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
2.7.1.162 | ATP + N-acetyl-alpha-D-galactosamine | Bifidobacterium longum | lacto-N-biose I/galacto-N-biose metabolic pathway | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
2.7.1.162 | ATP + N-acetyl-alpha-D-galactosamine | Bifidobacterium longum JCM 1217 | the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
2.7.1.162 | ATP + N-acetyl-alpha-D-galactosamine | Bifidobacterium longum JCM 1217 | lacto-N-biose I/galacto-N-biose metabolic pathway | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
2.7.1.162 | ATP + N-acetyl-alpha-D-glucosamine | Bifidobacterium longum | - |
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
2.7.1.162 | ATP + N-acetyl-alpha-D-glucosamine | Bifidobacterium longum | the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway | ADP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.211 | Bifidobacterium longum subsp. Longum | - |
- |
- |
2.4.1.211 | Bifidobacterium longum subsp. Longum JCM 1217 | - |
- |
- |
2.7.1.162 | Bifidobacterium longum | E8MF12 | strain JCM1217 | - |
2.7.1.162 | Bifidobacterium longum JCM 1217 | E8MF12 | strain JCM1217 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.162 | - |
Bifidobacterium longum |
2.7.1.162 | The transformant is cultivated and protein expression is induced by the addition of isopropyl-1-thio-beta-D-galactoside. The cells are harvested by centrifugation and sonicated. Cell debris is removed by centrifugation and enzyme is purified on an Ni-nitrilotriacetic acid agarose gel. | Bifidobacterium longum |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.1.162 | ATP + N-acetyl-D-hexosamine = ADP + N-acetyl-alpha-D-hexosamine 1-phosphate | enzyme acts by a sequential bi bi (two substrates-two products) mechanism, with the reaction occurring after the binding of both ATP and N-acetylhexosamine | Bifidobacterium longum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.162 | cell culture | strain JCM1217 | Bifidobacterium longum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.162 | additional information | - |
Incubation of 4 microM LnpB protein with 10 mM N-acetyl-alpha-D-glucosamine 6-sulfate in 0.1 M MOPS buffer (pH 7.5) at 30°C for 30 min shows that the protein have no hydrolytic activity | Bifidobacterium longum |
2.7.1.162 | 0.001 | - |
D-glucose, substrate donor specificity | Bifidobacterium longum |
2.7.1.162 | 0.002 | - |
D-mannosamine, substrate donor specificity | Bifidobacterium longum |
2.7.1.162 | 0.004 | - |
D-glucosamine, substrate donor specificity | Bifidobacterium longum |
2.7.1.162 | 0.007 | - |
2-deoxyglucose, substrate donor specificity | Bifidobacterium longum |
2.7.1.162 | 0.024 | - |
D-mannose, substrate donor specificity | Bifidobacterium longum |
2.7.1.162 | 0.049 | - |
D-talose, substrate donor specificity | Bifidobacterium longum |
2.7.1.162 | 0.22 | - |
N-acetyl-D-mannosamine, substrate donor specificity | Bifidobacterium longum |
2.7.1.162 | 0.48 | - |
ITP, substrate donor specificity | Bifidobacterium longum |
2.7.1.162 | 0.65 | - |
GTP, substrate donor specificity | Bifidobacterium longum |
2.7.1.162 | 0.89 | - |
N-acetyl-D-galactosamine, substrate donor specificity | Bifidobacterium longum |
2.7.1.162 | 1.48 | - |
ATP, substrate donor specificity | Bifidobacterium longum |
2.7.1.162 | 1.49 | - |
N-acetyl-D-glucosamine, substrate donor specificity | Bifidobacterium longum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.211 | beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate | the gene operon for LNBP lnpABCD encodes a lacto-N-biose I and beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine metabolic pathway with lacto-N-biose phosphorylase, N-acetylhexosamine 1-kinase, UDP-glucose hexose 1-phosphate uridylyltransferase, and UDP-glucose 4-epimerase, this pathway is involved in the intestinal colonization of bifidobacteria and utilizes lacto-N-biose I from human milk oligosaccharides or beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine from mucin sugars | Bifidobacterium longum subsp. Longum | alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine | - |
? | |
2.4.1.211 | beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate | the gene operon for LNBP lnpABCD encodes a lacto-N-biose I and beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine metabolic pathway with lacto-N-biose phosphorylase, N-acetylhexosamine 1-kinase, UDP-glucose hexose 1-phosphate uridylyltransferase, and UDP-glucose 4-epimerase, this pathway is involved in the intestinal colonization of bifidobacteria and utilizes lacto-N-biose I from human milk oligosaccharides or beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine from mucin sugars | Bifidobacterium longum subsp. Longum JCM 1217 | alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine | - |
? | |
2.4.1.211 | beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate | - |
Bifidobacterium longum subsp. Longum | alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine | - |
? | |
2.4.1.211 | beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate | - |
Bifidobacterium longum subsp. Longum JCM 1217 | alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine | - |
? | |
2.7.1.162 | ATP + D-mannose | phosphorylation at 1.6% of the activity with N-acetylglucosamine | Bifidobacterium longum | ? | - |
? | |
2.7.1.162 | ATP + D-mannose | phosphorylation at 1.6% of the activity with N-acetylglucosamine | Bifidobacterium longum JCM 1217 | ? | - |
? | |
2.7.1.162 | ATP + D-talose | phosphorylation at 3% of the activity with N-acetylglucosamine | Bifidobacterium longum | ? | - |
? | |
2.7.1.162 | ATP + N-acetyl-alpha-D-galactosamine | the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway | Bifidobacterium longum | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
2.7.1.162 | ATP + N-acetyl-alpha-D-galactosamine | phosphorylation at 60% of the activity with N-acetylglucosamine | Bifidobacterium longum | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
2.7.1.162 | ATP + N-acetyl-alpha-D-galactosamine | lacto-N-biose I/galacto-N-biose metabolic pathway | Bifidobacterium longum | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
2.7.1.162 | ATP + N-acetyl-alpha-D-galactosamine | the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway | Bifidobacterium longum JCM 1217 | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
2.7.1.162 | ATP + N-acetyl-alpha-D-galactosamine | phosphorylation at 60% of the activity with N-acetylglucosamine | Bifidobacterium longum JCM 1217 | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
2.7.1.162 | ATP + N-acetyl-alpha-D-galactosamine | lacto-N-biose I/galacto-N-biose metabolic pathway | Bifidobacterium longum JCM 1217 | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
2.7.1.162 | ATP + N-acetyl-alpha-D-glucosamine | - |
Bifidobacterium longum | ADP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
2.7.1.162 | ATP + N-acetyl-alpha-D-glucosamine | the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway | Bifidobacterium longum | ADP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
2.7.1.162 | ATP + N-acetyl-D-mannosamine | phosphorylation at 15% of the activity with N-acetylglucosamine | Bifidobacterium longum | ? | - |
? | |
2.7.1.162 | ATP + N-acetyl-D-mannosamine | - |
Bifidobacterium longum | ADP + ? | - |
? | |
2.7.1.162 | GTP + N-acetyl-alpha-D-glucosamine | GTP shows about 45% of the activity with ATP | Bifidobacterium longum | GDP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
2.7.1.162 | ITP + N-acetyl-alpha-D-glucosamine | ITP shows about 30% of the activity with ATP | Bifidobacterium longum | IDP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
2.7.1.162 | additional information | slightly active with D-talose, D-mannose, GTP, ITP | Bifidobacterium longum | ? | - |
? | |
2.7.1.162 | additional information | slightly active with D-talose, D-mannose, GTP, ITP | Bifidobacterium longum JCM 1217 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.211 | 1,3-beta-galactosyl-N-acetylhexosamine phosphorylase | - |
Bifidobacterium longum subsp. Longum |
2.4.1.211 | lacto-N-biose phosphorylase | - |
Bifidobacterium longum subsp. Longum |
2.4.1.211 | lnba | - |
Bifidobacterium longum subsp. Longum |
2.4.1.211 | LNBP | - |
Bifidobacterium longum subsp. Longum |
2.7.1.162 | lnpB | - |
Bifidobacterium longum |
2.7.1.162 | lnpB | protein | Bifidobacterium longum |
2.7.1.162 | N-acetylhexosamine 1-kinase | - |
Bifidobacterium longum |
2.7.1.162 | NahK | - |
Bifidobacterium longum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.162 | 40 | - |
- |
Bifidobacterium longum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.162 | 50 | - |
pH 8.5, 30 min, 50% loss of activity | Bifidobacterium longum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.162 | 0.752 | - |
N-acetyl-alpha-D-galactosamine | - |
Bifidobacterium longum | |
2.7.1.162 | 1.21 | - |
N-acetyl-alpha-D-glucosamine | - |
Bifidobacterium longum | |
2.7.1.162 | 1.21 | - |
N-acetyl-alpha-D-glucosamine | pH 8.5, 30°C | Bifidobacterium longum | |
2.7.1.162 | 6.08 | - |
N-acetyl-alpha-D-galactosamine | pH 8.5, 30°C | Bifidobacterium longum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.162 | 8.5 | - |
- |
Bifidobacterium longum |
2.7.1.162 | 8.5 | - |
assay at | Bifidobacterium longum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.162 | 5 | 9.5 | stable | Bifidobacterium longum |
2.7.1.162 | 5 | 9.5 | stability at 30°C | Bifidobacterium longum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.162 | ATP | - |
Bifidobacterium longum | |
2.7.1.162 | GTP | - |
Bifidobacterium longum | |
2.7.1.162 | ITP | - |
Bifidobacterium longum |