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Literature summary extracted from

  • Yoshida, S.; Atomi, H.; Imanaka, T.
    Engineering of a type III Rubisco from a hyperthermophilic archaeon in order to enhance catalytic performance in mesophilic host cells (2007), Appl. Environ. Microbiol., 73, 6254-6261.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.39 additional information a solution containing 10 mM NaHCO3, 10 mM MgCl2, and 10 mM Bicine-NaOH (pH 8.3) is used for enzyme activation Thermococcus kodakarensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.39 expressed in Escherichia coli BL21(DE3)CodonPlus RIL cells Thermococcus kodakarensis

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.39 additional information Mutants SP1 and SP2 harbor mutations in the nonconserved residues of loop 6 leading to strongly decreased specific activities. Within alpha-helix 6, SP3 contains a single amino acid substitution from valine to threonine leading to increased specific activity. Mutant SP4 has substitutions in five consecutive amino acid residues leading to increased specific activity. SP5 has a 6-amino-acid substitution in alpha-helix 6 leading to decreased specific activity, while SP6 has 11 substitutions in alpha-helix 6 leading to 31% increased specific activity. In SP7, the entire loop 6/alpha-helix 6 region is replaced with the corresponding region of spinach Rubisco resulting in strongly depressed specific activity. Thermococcus kodakarensis
4.1.1.39 V330T negative effect on Rbc activity Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.39 0.052
-
 CO2 wild type enzyme, at 25°C Thermococcus kodakarensis
4.1.1.39 0.9
-
 O2 wild type enzyme, at 25°C Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.39 Thermococcus kodakarensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.39 PD-10 gel filtration Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.39 D-ribulose 1,5-bisphosphate + CO2 + H2O
-
 Thermococcus kodakarensis 3-phospho-D-glycerate
-
 ?
4.1.1.39 D-ribulose 1,5-bisphosphate + O2
-
 Thermococcus kodakarensis 3-phospho-D-glycerate + 2-phosphoglycolate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.39 Rbc
-
 Thermococcus kodakarensis
4.1.1.39 Rubisco
-
 Thermococcus kodakarensis
4.1.1.39 type III ribulose 1,5-bisphosphate carboxylase/oxygenase
-
 Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.39 90 100 the half life of the wild type enzyme is about 5fold lower at 100°C than at 90°C Thermococcus kodakarensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.39 0.25
-
 CO2 wild type enzyme, at 25°C Thermococcus kodakarensis
4.1.1.39 0.38
-
 O2 wild type enzyme, at 25°C Thermococcus kodakarensis