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Literature summary extracted from

  • Lagaert, S.; Van Campenhout, S.; Pollet, A.; Bourgois, T.M.; Delcour, J.A.; Courtin, C.M.; Volckaert, G.
    Recombinant expression and characterization of a reducing-end xylose-releasing exo-oligoxylanase from Bifidobacterium adolescentis (2007), Appl. Environ. Microbiol., 73, 5374-5377.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.156 expressed as a His-tagged fusion protein in Escherichia coli Bifidobacterium adolescentis

General Stability

EC Number General Stability Organism
3.2.1.156 purified enzyme is stable for at least 4 months at 4°C in 25 mM sodium acetate buffer, pH 5.0 Bifidobacterium adolescentis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.156 42000
-
 SDS-PAGE Bifidobacterium adolescentis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.156 Bifidobacterium adolescentis A1A048 putative
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.156 by using a single-step HisTrap purification performed on a Atka fast protein liquid chromatograph. Protein yield: 140 mg recombinant enzyme per liter cell culture Bifidobacterium adolescentis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.156 xylopentaose + H2O the recombinant enzyme constitutively hydrolyzes a xylose residue from Xn when n is above 2, until the final products are (n-2)X1 and X2 Bifidobacterium adolescentis xylotetraose + xylobiose
-
 ?
3.2.1.156 xylotetraose + H2O
-
 Bifidobacterium adolescentis xylotriose + xylobiose
-
 ?
3.2.1.156 xylotriose + H2O
-
 Bifidobacterium adolescentis D-xylose + xylobiose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.156 RexA
-
 Bifidobacterium adolescentis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.156 40
-
-
 Bifidobacterium adolescentis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.156 40
-
 RexA is stable at temperatures up to 40°C Bifidobacterium adolescentis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.156 6
-
-
 Bifidobacterium adolescentis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.156 4 10
-
 Bifidobacterium adolescentis