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Literature summary extracted from

  • Sekine, T.; Kawaguchi, A.; Hamano, Y.; Takagi, H.
    Desensitization of feedback inhibition of the Saccharomyces cerevisiae gamma-glutamyl kinase enhances proline accumulation and freezing tolerance (2007), Appl. Environ. Microbiol., 73, 4011-4019.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.2.11 additional information GK activity requires gamma-glutamyl phosphate reductase Saccharomyces cerevisiae

Application

EC Number Application Comment Organism
2.7.2.11 additional information GK is the rate-limiting enzyme in the proline biosynthesis Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.11 expression of PRO1 in Escherichia coli M15(pREP4) Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.7.2.11 D154N is 64fold less sensitive to feedback inhibition than wild-type GK, leading to proline accumulation Saccharomyces cerevisiae
2.7.2.11 E149K very insensitive to feedback inhibition, shows prominent increase in the proline content, freeze tolerance like the D154N mutant Saccharomyces cerevisiae
2.7.2.11 I150T very insensitive to feedback inhibition, shows prominent increase in the proline content, is more tolerant to freezing stress than cells expressing the D154N mutant Saccharomyces cerevisiae
2.7.2.11 N142D/I166V very insensitive to feedback inhibition, shows prominent increase in the proline content, is more tolerant to freezing stress than cells expressing the D154N mutant Saccharomyces cerevisiae
2.7.2.11 S146P shows prominent increase in the proline content Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.11 L-proline GK activity is subject to feedback inhibition by proline Saccharomyces cerevisiae
2.7.2.11 additional information no significant differences in growth between mutant yeast cells on SD agar medium with heat shock (50°C, 3 to 5 h), ethanol (16 to20%), and sorbitol (2.0 to 2.5 M) stresses, although the proline-accumulating cells are more resistant to these stresses than cells expressing wild-type GK Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.11 47000
-
gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.11 Saccharomyces cerevisiae P32264 strain INVDput1pro1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.11 by Ni2+ column and gel filtration Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.11 3.2
-
mutant N142D/I166V Saccharomyces cerevisiae
2.7.2.11 4.2
-
mutant E149K Saccharomyces cerevisiae
2.7.2.11 14.7
-
wild-type Saccharomyces cerevisiae
2.7.2.11 26.3
-
mutant D154N Saccharomyces cerevisiae
2.7.2.11 29
-
mutant I150T Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.11 ATP + L-glutamate
-
Saccharomyces cerevisiae ADP + L-glutamate 5-phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.2.11 gamma-glutamyl kinase
-
Saccharomyces cerevisiae
2.7.2.11 GK
-
Saccharomyces cerevisiae

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.2.11 0.5
-
wild-type Saccharomyces cerevisiae L-proline
2.7.2.11 32
-
mutant D154N Saccharomyces cerevisiae L-proline
2.7.2.11 50
-
mutant I150T Saccharomyces cerevisiae L-proline
2.7.2.11 54
-
mutant N142D/I166V Saccharomyces cerevisiae L-proline
2.7.2.11 840
-
mutant E149K Saccharomyces cerevisiae L-proline