EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.2.11 | additional information | GK activity requires gamma-glutamyl phosphate reductase | Saccharomyces cerevisiae |
EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.2.11 | additional information | GK is the rate-limiting enzyme in the proline biosynthesis | Saccharomyces cerevisiae |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.2.11 | expression of PRO1 in Escherichia coli M15(pREP4) | Saccharomyces cerevisiae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.2.11 | D154N | is 64fold less sensitive to feedback inhibition than wild-type GK, leading to proline accumulation | Saccharomyces cerevisiae |
2.7.2.11 | E149K | very insensitive to feedback inhibition, shows prominent increase in the proline content, freeze tolerance like the D154N mutant | Saccharomyces cerevisiae |
2.7.2.11 | I150T | very insensitive to feedback inhibition, shows prominent increase in the proline content, is more tolerant to freezing stress than cells expressing the D154N mutant | Saccharomyces cerevisiae |
2.7.2.11 | N142D/I166V | very insensitive to feedback inhibition, shows prominent increase in the proline content, is more tolerant to freezing stress than cells expressing the D154N mutant | Saccharomyces cerevisiae |
2.7.2.11 | S146P | shows prominent increase in the proline content | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.2.11 | L-proline | GK activity is subject to feedback inhibition by proline | Saccharomyces cerevisiae | |
2.7.2.11 | additional information | no significant differences in growth between mutant yeast cells on SD agar medium with heat shock (50°C, 3 to 5 h), ethanol (16 to20%), and sorbitol (2.0 to 2.5 M) stresses, although the proline-accumulating cells are more resistant to these stresses than cells expressing wild-type GK | Saccharomyces cerevisiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.2.11 | 47000 | - |
gel filtration | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.2.11 | Saccharomyces cerevisiae | P32264 | strain INVDput1pro1 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.2.11 | by Ni2+ column and gel filtration | Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.2.11 | 3.2 | - |
mutant N142D/I166V | Saccharomyces cerevisiae |
2.7.2.11 | 4.2 | - |
mutant E149K | Saccharomyces cerevisiae |
2.7.2.11 | 14.7 | - |
wild-type | Saccharomyces cerevisiae |
2.7.2.11 | 26.3 | - |
mutant D154N | Saccharomyces cerevisiae |
2.7.2.11 | 29 | - |
mutant I150T | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.11 | ATP + L-glutamate | - |
Saccharomyces cerevisiae | ADP + L-glutamate 5-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.2.11 | gamma-glutamyl kinase | - |
Saccharomyces cerevisiae |
2.7.2.11 | GK | - |
Saccharomyces cerevisiae |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.7.2.11 | 0.5 | - |
wild-type | Saccharomyces cerevisiae | L-proline | |
2.7.2.11 | 32 | - |
mutant D154N | Saccharomyces cerevisiae | L-proline | |
2.7.2.11 | 50 | - |
mutant I150T | Saccharomyces cerevisiae | L-proline | |
2.7.2.11 | 54 | - |
mutant N142D/I166V | Saccharomyces cerevisiae | L-proline | |
2.7.2.11 | 840 | - |
mutant E149K | Saccharomyces cerevisiae | L-proline |