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Literature summary extracted from

  • Arima, J.; Uesugi, Y.; Iwabuchi, M.; Hatanaka, T.
    Change in substrate preference of Streptomyces aminopeptidase through modification of the environment around the substrate binding site (2006), Appl. Environ. Microbiol., 72, 7962-7967.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.10 expressed in Escherichia coli BL21(DE3) cells Streptomyces septatus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.10 D198A/F221T decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Phe-p-nitroanilide Streptomyces septatus
3.4.11.10 D198F/F221D decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Arg-p-nitroanilide Streptomyces septatus
3.4.11.10 D198H/F221N decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Phe-p-nitroanilide Streptomyces septatus
3.4.11.10 D198I/F221W decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-cystinyl-p-nitroanilide Streptomyces septatus
3.4.11.10 D198L/F221E decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Arg-p-nitroanilide Streptomyces septatus
3.4.11.10 D198L/F221M decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Phe-p-nitroanilide Streptomyces septatus
3.4.11.10 D198L/F221T decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Phe-p-nitroanilide Streptomyces septatus
3.4.11.10 D198L/F221W decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-cystinyl-p-nitroanilide Streptomyces septatus
3.4.11.10 D198M/F221A decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Phe-p-nitroanilide Streptomyces septatus
3.4.11.10 D198M/F221G decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Phe-p-nitroanilide Streptomyces septatus
3.4.11.10 D198M/F221V decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Phe-p-nitroanilide Streptomyces septatus
3.4.11.10 D198M/F221W highest activity towards L-Leu-p-nitroanilide like the wild type enzyme Streptomyces septatus
3.4.11.10 D198P/F221E decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Arg-p-nitroanilide Streptomyces septatus
3.4.11.10 D198S/F221E decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Arg-p-nitroanilide Streptomyces septatus
3.4.11.10 D198S/F221N decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Phe-p-nitroanilide Streptomyces septatus
3.4.11.10 D198V/F221D decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Lys-p-nitroanilide Streptomyces septatus
3.4.11.10 D198V/F221N decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Phe-p-nitroanilide Streptomyces septatus
3.4.11.10 D198W/F221D decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Arg-p-nitroanilide Streptomyces septatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.10 0.056
-
 L-cystinyl-p-nitroanilide mutant enzyme D198I/F221W, at pH 8.0 and 37°C Streptomyces septatus
3.4.11.10 0.11
-
 L-cystinyl-p-nitroanilide wild type enzyme, at pH 8.0 and 37°C Streptomyces septatus
3.4.11.10 0.57
-
 L-Leu-p-nitroanilide wild type enzyme, at pH 8.0 and 37°C Streptomyces septatus
3.4.11.10 1
-
 L-Arg-p-nitroanilide wild type enzyme, at pH 8.0 and 37°C Streptomyces septatus
3.4.11.10 1.15
-
 L-Leu-p-nitroanilide mutant enzyme D198F/F221I, at pH 8.0 and 37°C Streptomyces septatus
3.4.11.10 7.51
-
 L-Arg-p-nitroanilide mutant enzyme D198P/F221E, at pH 8.0 and 37°C Streptomyces septatus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.10 Streptomyces septatus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.10 22
-
 mutant enzyme D198M/F221W, using L-Leu-p-nitroanilide as a substrate Streptomyces septatus
3.4.11.10 68
-
 wild type enzyme, using L-Leu-p-nitroanilide as a substrate Streptomyces septatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.10 L-Ala-p-nitroanilide + H2O 1% activity compared to L-Leu-p-nitroanilide Streptomyces septatus L-Ala + p-nitroaniline
-
 ?
3.4.11.10 L-Arg-p-nitroanilide + H2O 3% activity compared to L-Leu-p-nitroanilide Streptomyces septatus L-Arg + p-nitroaniline
-
 ?
3.4.11.10 L-cystinyl-p-nitroanilide + H2O 10% activity compared to L-Leu-p-nitroanilide Streptomyces septatus L-cystine + p-nitroaniline
-
 ?
3.4.11.10 L-Leu-p-nitroanilide + H2O 100% activity Streptomyces septatus L-Leu + p-nitroaniline
-
 ?
3.4.11.10 L-Lys-p-nitroanilide + H2O 24% activity compared to L-Leu-p-nitroanilide Streptomyces septatus L-Lys + p-nitroaniline
-
 ?
3.4.11.10 L-Phe-p-nitroanilide + H2O 23% activity compared to L-Leu-p-nitroanilide Streptomyces septatus L-Phe + p-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.10 SSAP
-
 Streptomyces septatus
3.4.11.10 TH-2
-
 Streptomyces septatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.10 1.26
-
 L-Arg-p-nitroanilide wild type enzyme, at pH 8.0 and 37°C Streptomyces septatus
3.4.11.10 3.71
-
 L-cystinyl-p-nitroanilide wild type enzyme, at pH 8.0 and 37°C Streptomyces septatus
3.4.11.10 41.3
-
 L-Leu-p-nitroanilide wild type enzyme, at pH 8.0 and 37°C Streptomyces septatus
3.4.11.10 46.7
-
 L-cystinyl-p-nitroanilide mutant enzyme D198I/F221W, at pH 8.0 and 37°C Streptomyces septatus
3.4.11.10 82
-
 L-Arg-p-nitroanilide mutant enzyme D198P/F221E, at pH 8.0 and 37°C Streptomyces septatus
3.4.11.10 128
-
 L-Leu-p-nitroanilide mutant enzyme D198F/F221I, at pH 8.0 and 37°C Streptomyces septatus