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Literature summary extracted from

  • Zhang, J.Y.; Zou, J.; Bao, Q.; Chen, W.L.; Wang, L.; Yang, H.; Zhang, C.C.
    A lithium-sensitive and sodium-tolerant 3-phosphoadenosine-5-phosphatase encoded by halA from the cyanobacterium Arthrospira platensis is closely related to its counterparts from yeasts and plants (2006), Appl. Environ. Microbiol., 72, 245-251.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.7 expressed in Escherichia coli Limnospira platensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.7 Li+
-
 Limnospira platensis
3.1.3.7 Na+
-
 Limnospira platensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.7 0.61
-
 adenosine 3'-phosphate 5'-phosphosulfate in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30°C Limnospira platensis
3.1.3.7 1.03
-
 adenosine 3',5'-bisphosphate in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30°C Limnospira platensis
3.1.3.7 41.5
-
 5'-ADP in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30°C Limnospira platensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.7 Mg2+ the activity increases over increasing concentrations of Mg2+ to reach its optimal values in the presence of 2 to 2.5 mM Mg2+ Limnospira platensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.7 41800
-
 SDS-PAGE Limnospira platensis

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.7 Limnospira platensis Q3LS17 formerly known as Arthrospira platensis
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.7 nickel affinity chromatography Limnospira platensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.7 5'-ADP + H2O
-
 Limnospira platensis ?
-
 ?
3.1.3.7 adenosine 3',5'-bisphosphate + H2O 100% activity Limnospira platensis adenosine 5'-phosphate + phosphate
-
 ?
3.1.3.7 adenosine 3'-phosphate 5'-phosphosulfate + H2O 42% activity compared to adenosine 3',5'bisphosphate Limnospira platensis adenosine 5'-phosphosulfate + phosphate
-
 ?
3.1.3.7 inositol-1,4-bisphosphate + H2O 5.6% activity compared to adenosine 3',5'bisphosphate Limnospira platensis inositol 4-phosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.7 3-phosphoadenosine-5-phosphatase
-
 Limnospira platensis
3.1.3.7 HalA
-
 Limnospira platensis
3.1.3.7 PAPase
-
 Limnospira platensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.7 8
-
-
 Limnospira platensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.7 10
-
 remained 60% of maximal activity when the pH of the reaction sample is increased to pH 10 Limnospira platensis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.7 3.6
-
 in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30°C Limnospira platensis Li+
3.1.3.7 600
-
 in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30°C Limnospira platensis Na+