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Literature summary extracted from

  • Carpinelli, J.; Kraemer, R.; Agosin, E.
    Metabolic engineering of Corynebacterium glutamicum for trehalose overproduction: role of the TreYZ trehalose biosynthetic pathway (2006), Appl. Environ. Microbiol., 72, 1949-1955.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.141 food industry trehalose has many potential applications in biotechnology and the food industry due to its protective effect against environmental stress. Raising trehalose productivity can be achieved through homologous overexpression of maltooligosyltrehalose synthase and maltooligosyltrehalose trehalohydrolase. Overexpression of the treY gene and the treY/treZ synthetic operon significantly increases maltooligosyltrehalose synthase activity, the rate-limiting step, and improves the specific productivity and the final titer of trehalose. Furthermore, a strong decrease is noted in glycogen accumulation. Expression of galU/treY and galU/treYZ synthetic operons show a partial recovery in the intracellular glycogen levels and a significant improvement in both intra- and extracellular trehalose content Corynebacterium glutamicum
5.4.99.15 synthesis overexpression of the enzyme treY gene and the treY/treZ synthetic operon encoding enzyme and maltooligosyltrehalose trehalohydrolase significantly increases maltooligosyltrehalose synthase activity, the rate-limiting step, and improves the specific productivity and the final titer of trehalose. Furthermore, a strong decrease is noted in glycogen accumulation. Expression of UDP-glucose pyrophosphorylase/maltooligosyltrehalose synthase and UDP-glucose pyrophosphorylase/maltooligosyltrehalose synthase/maltooligosyltrehalose trehalohydrolase synthetic operons shows a partial recovery in the intracellular glycogen levels and a significant improvement in both intra- and extracellular trehalose content Corynebacterium glutamicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.141 overexpression of the treZ gene and the treY/treZ synthetic operon Corynebacterium glutamicum
5.4.99.15 overexpression of enzyme and maltooligosyltrehalose trehalohydrolase alone or with UPD-glucose pyrophosphorylase in a synthetic operon to improve trehalose production in Escherichia coli Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.141 Corynebacterium glutamicum
-
-
-
5.4.99.15 Corynebacterium glutamicum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.141 maltoheptaosyltrehalose + H2O
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Corynebacterium glutamicum maltoheptaose + alpha,alpha'-trehalose
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.141 Maltooligosyl trehalose trehalohydrolase
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Corynebacterium glutamicum
3.2.1.141 TreZ
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Corynebacterium glutamicum