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Literature summary extracted from

  • Lianghua, T.; Liming, X.; Min, S.; Huaying, G.
    Purification and application of a lipase from Penicillium expansum PED-03 (2007), Appl. Biochem. Biotechnol., 142, 194-199.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.3 synthesis the enzyme shows potential in the production of optically pure ibuprofen Penicillium expansum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular
-
 Penicillium expansum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 28000
-
 x * 28000, SDS-PAGE Penicillium expansum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Penicillium expansum Q7LST4 precursor; isolated from the waste of a rap oil manufactory in China
-
3.1.1.3 Penicillium expansum PED-03 Q7LST4 precursor; isolated from the waste of a rap oil manufactory in China
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 native extracellular lipase 81.8fold from fermentation broth by anion exchange chromatography and gel filtration Penicillium expansum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 cell culture
-
 Penicillium expansum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 85.94
-
 purified extracellular lipase Penicillium expansum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 (R,S)-ibuprofen + 1-propanol purified enzyme in a mixture of 1-propanol-isooctane in a ratio of 3:1, the conversion of racemic ibuprofen reaches 46% with very high enantioselectivity for production of the (S)-enantiomer Penicillium expansum (S)-ibuprofen n-propyl ester + (R)-ibuprofen
-
 ?
3.1.1.3 (R,S)-ibuprofen + 1-propanol purified enzyme in a mixture of 1-propanol-isooctane in a ratio of 3:1, the conversion of racemic ibuprofen reaches 46% with very high enantioselectivity for production of the (S)-enantiomer Penicillium expansum PED-03 (S)-ibuprofen n-propyl ester + (R)-ibuprofen
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Penicillium expansum 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Penicillium expansum PED-03 4-nitrophenol + palmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 28000, SDS-PAGE Penicillium expansum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 37
-
 assay at Penicillium expansum
3.1.1.3 55
-
 conversion of racemic ibuprofen Penicillium expansum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
 assay at Penicillium expansum