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Literature summary extracted from

  • Louime, C.; Abazinge, M.; Johnson, E.; Latinwo, L.; Ikediobi, C.; Clark, A.M.
    Molecular cloning and biochemical characterization of a family-9 endoglucanase with an unusual structure from the gliding bacteria Cytophaga hutchinsonii (2007), Appl. Biochem. Biotechnol., 141, 127-138.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 overexpression in Escherichia coli with a His-Tag based expression vector Cytophaga hutchinsonii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Cytophaga hutchinsonii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Cytophaga hutchinsonii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O Cel9A is a nonprocessive enzyme with endo-cellulase activities Cytophaga hutchinsonii ?
-
 ?
3.2.1.4 additional information Cel9A does not cleave xylan Cytophaga hutchinsonii ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 Cel9A
-
 Cytophaga hutchinsonii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 45
-
-
 Cytophaga hutchinsonii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 40 50 stable Cytophaga hutchinsonii
3.2.1.4 60
-
 30% loss of activity overnight Cytophaga hutchinsonii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6 6.5
-
 Cytophaga hutchinsonii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 5 9 pH 5.0: about 40% of maximal activity, pH 9.0: about 55% of maximal activity Cytophaga hutchinsonii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.4 Cytophaga hutchinsonii calculated from sequence
-
 6.5