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Literature summary extracted from

  • O'Connell, S.; Walsh, G.
    Purification and properties of a beta-galactosidase with potential application as a digestive supplement (2007), Appl. Biochem. Biotechnol., 141, 1-14.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.23 medicine use of enzyme as a digestive supplement Kluyveromyces marxianus
3.2.1.23 nutrition the enzyme has a potential application as a digestive supplement Kluyveromyces marxianus
3.2.1.23 nutrition use of enzyme as a digestive supplement Kluyveromyces marxianus

General Stability

EC Number General Stability Organism
3.2.1.23 the unpurified beta-galactosidase displays a high level of stability when exposed to simulated intestinal conditions in vitro for 4 h Kluyveromyces marxianus
3.2.1.23 the unpurified enzyme displays a high level of stability when exposed to simulated intestinal conditions in vitro for 4 h Kluyveromyces marxianus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 D-galactose 5 mM, 93% residual activity Kluyveromyces marxianus
3.2.1.23 D-glucose 5 mM, 86% residual activity Kluyveromyces marxianus
3.2.1.23 isopropyl beta-D-thiogalactoside 5 mM, 91% residual activity Kluyveromyces marxianus
3.2.1.23 additional information no or poor inhibition by D-glucose, D-galactose, IPTG, and sucrose; no or poor inhibition by D-glucose, D-galactose, IPTG, and sucrose Kluyveromyces marxianus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 4
-
 lactose pH 6.8 Kluyveromyces marxianus
3.2.1.23 4
-
 lactose pH 6.8, 37°C Kluyveromyces marxianus
3.2.1.23 4.1
-
 2-nitrophenyl-beta-D-galactopyranoside pH 6.8, 37°C Kluyveromyces marxianus
3.2.1.23 4.7
-
 2-nitrophenyl-beta-D-galactoside pH 6.8 Kluyveromyces marxianus
3.2.1.23 7.1
-
 4-nitrophenyl-beta-D-galactoside pH 6.8 Kluyveromyces marxianus
3.2.1.23 7.1
-
 4-nitrophenyl-beta-D-galactopyranoside pH 6.8, 37°C Kluyveromyces marxianus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 123000
-
 2 * 123000, SDS-PAGE Kluyveromyces marxianus
3.2.1.23 251000
-
 gel filtration Kluyveromyces marxianus
3.2.1.23 251000
-
 PAGE Kluyveromyces marxianus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.23 lactose + H2O Kluyveromyces marxianus the enzyme is involved in lactose hydrolysis in the small intestine of the host organism D-glucose + D-galactose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Kluyveromyces marxianus
-
 strain DSM5418
-
3.2.1.23 Kluyveromyces marxianus P00723 strain DSM 5418
-
3.2.1.23 Kluyveromyces marxianus Q6QTF4 strain DSM 5418
-
3.2.1.23 Kluyveromyces marxianus DSM 5418
-
 strain DSM5418
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.23 no glycoprotein
-
 Kluyveromyces marxianus
3.2.1.23 proteolytic modification pancreatin activates the beta-galactosidase due to a partial proteolytic cleavage yielding a more active enzyme form Kluyveromyces marxianus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Kluyveromyces marxianus
3.2.1.23 native enzyme 115fold to homogeneity by gel filtration, and anion exchange and hydroxylapatite chromatography Kluyveromyces marxianus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 additional information
-
-
 Kluyveromyces marxianus
3.2.1.23 790
-
 pH 6.8 Kluyveromyces marxianus
3.2.1.23 790.6
-
 purified enzyme Kluyveromyces marxianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Kluyveromyces marxianus 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactoside + H2O
-
 Kluyveromyces marxianus 2-nitrophenol + D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactoside + H2O
-
 Kluyveromyces marxianus DSM 5418 2-nitrophenol + D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Kluyveromyces marxianus 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactoside + H2O
-
 Kluyveromyces marxianus 4-nitrophenol + D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactoside + H2O
-
 Kluyveromyces marxianus DSM 5418 4-nitrophenol + D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Kluyveromyces marxianus D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O the enzyme is involved in lactose hydrolysis in the small intestine of the host organism Kluyveromyces marxianus D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Kluyveromyces marxianus D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Kluyveromyces marxianus DSM 5418 D-galactose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 dimer 2 * 123000, SDS-PAGE Kluyveromyces marxianus
3.2.1.23 More peptide mass fingerprinting Kluyveromyces marxianus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 37
-
-
 Kluyveromyces marxianus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.23 22 50 temperature profile, about 50% of maximal activity between 20°C and 45°C Kluyveromyces marxianus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.23 6300
-
 lactose pH 6.8 Kluyveromyces marxianus
3.2.1.23 6300
-
 lactose pH 6.8, 37°C Kluyveromyces marxianus
3.2.1.23 8559
-
 2-nitrophenyl-beta-D-galactoside pH 6.8 Kluyveromyces marxianus
3.2.1.23 8559
-
 2-nitrophenyl-beta-D-galactopyranoside pH 6.8, 37°C Kluyveromyces marxianus
3.2.1.23 270000
-
 4-nitrophenyl-beta-D-galactopyranoside pH 6.8, 37°C Kluyveromyces marxianus
3.2.1.23 2700000
-
 4-nitrophenyl-beta-D-galactoside pH 6.8 Kluyveromyces marxianus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 6.8
-
-
 Kluyveromyces marxianus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.23 3.5 10 about 50% of maximal activity between pH 5.6 and pH 7.8 Kluyveromyces marxianus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.23 Kluyveromyces marxianus isoelectric focusing
-
 5.1