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Literature summary extracted from

  • Hirasawa, K.; Uchimura, K.; Kashiwa, M.; Grant, W.D.; Ito, S.; Kobayashi, T.; Horikoshi, K.
    Salt-activated endoglucanase of a strain of alkaliphilic Bacillus agaradhaerens (2006), Antonie van Leeuwenhoek, 89, 211-219.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.4 CH3COONa 1.6 M, 4.45fold activation Salipaludibacillus agaradhaerens
3.2.1.4 NH4Cl 0.2 M, 3fold activation Salipaludibacillus agaradhaerens
3.2.1.4 NH4NO3 0.05 M, 2.8fold activation Salipaludibacillus agaradhaerens

General Stability

EC Number General Stability Organism
3.2.1.4 enzyme is stable to incubation with hydrogen peroxide Salipaludibacillus agaradhaerens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Fe2+ 2 mM, 67% inhibition Salipaludibacillus agaradhaerens
3.2.1.4 Hg2+ 2 mM, 20% inhibition Salipaludibacillus agaradhaerens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Ca2+ 2 mM, 1.5fold activation Salipaludibacillus agaradhaerens
3.2.1.4 KCl 0.8 M, 4.23fold activation Salipaludibacillus agaradhaerens
3.2.1.4 LiCl 0.05 M, 1.9fold activation Salipaludibacillus agaradhaerens
3.2.1.4 Mg2+ 2 mM, 1.48fold activation Salipaludibacillus agaradhaerens
3.2.1.4 Na2SO4 0.5 M, 4.43fold activation Salipaludibacillus agaradhaerens
3.2.1.4 NaBr 0.5 M, 3.84fold activation Salipaludibacillus agaradhaerens
3.2.1.4 NaCl the enzyme activity increass around 4fold by the addition of 0.2-2.0 M NaCl in 0.1 M glycine-NaOH buffer (pH 9.0) Salipaludibacillus agaradhaerens
3.2.1.4 NaNO3 0.5 M, 4fold activation Salipaludibacillus agaradhaerens
3.2.1.4 Sr2+ 2 mM, 1.45fold activation Salipaludibacillus agaradhaerens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 38000
-
 x * 38000, SDS-PAGE Salipaludibacillus agaradhaerens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Salipaludibacillus agaradhaerens
-
 JAM-KU023
-
3.2.1.4 Salipaludibacillus agaradhaerens JAM-KU023
-
 JAM-KU023
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Salipaludibacillus agaradhaerens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.4 culture medium
-
 Salipaludibacillus agaradhaerens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 avicel + H2O 33% of the activity with carboxymethyl cellulose Salipaludibacillus agaradhaerens ?
-
 ?
3.2.1.4 avicel + H2O 33% of the activity with carboxymethyl cellulose Salipaludibacillus agaradhaerens JAM-KU023 ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Salipaludibacillus agaradhaerens ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Salipaludibacillus agaradhaerens JAM-KU023 ?
-
 ?
3.2.1.4 lichenan + H2O 380% of the activity with carboxymethyl cellulose Salipaludibacillus agaradhaerens ?
-
 ?
3.2.1.4 lichenan + H2O 380% of the activity with carboxymethyl cellulose Salipaludibacillus agaradhaerens JAM-KU023 ?
-
 ?
3.2.1.4 additional information the enzyme does not degrade curdran, xylan, laminarin and pectin (0.5% each) at all Salipaludibacillus agaradhaerens ?
-
 ?
3.2.1.4 additional information the enzyme does not degrade curdran, xylan, laminarin and pectin (0.5% each) at all Salipaludibacillus agaradhaerens JAM-KU023 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 38000, SDS-PAGE Salipaludibacillus agaradhaerens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 50
-
 optimum without NaCl. Enzyme activity increases approximately 6fold at 60°C with 0.2 M NaCl compared to that at 50°C without NaCl in 0.1 M glycine-NaOH buffer (pH 9.0) Salipaludibacillus agaradhaerens
3.2.1.4 60
-
 optimum with 0.2 M NaCl. Enzyme activity increases approximately 6fold at 60°C with 0.2 M NaCl compared to that at 50°C without NaCl in 0.1 M glycine-NaOH buffer (pH 9.0) Salipaludibacillus agaradhaerens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.4 7.4
-
 carboxymethyl cellulose in presence of 25 mM Salipaludibacillus agaradhaerens
3.2.1.4 84.4
-
 carboxymethyl cellulose in presence of 250 mM Salipaludibacillus agaradhaerens
3.2.1.4 190.1
-
 carboxymethyl cellulose in presence of 25 mM Salipaludibacillus agaradhaerens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6.5 7 optimum without NaCl Salipaludibacillus agaradhaerens
3.2.1.4 7 9.4 optimum with 0.2 M NaC Salipaludibacillus agaradhaerens