Any feedback?
Literature summary extracted from
- Development of a high-throughput purification method and a continuous assay system for chlorophyllase (2006), Anal. Biochem., 353, 93-98.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.14 | expression as His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Triticum aestivum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.14 | 4-hydroxymercuribenzoate | - |
Triticum aestivum |  |
| 3.1.1.14 | Cu2+ | - |
Triticum aestivum | |
| 3.1.1.14 | diisopropyl fluorophosphate | - |
Triticum aestivum | |
| 3.1.1.14 | Fe2+ | - |
Triticum aestivum | |
| 3.1.1.14 | Fe3+ | - |
Triticum aestivum | |
| 3.1.1.14 | Hg2+ | - |
Triticum aestivum | |
| 3.1.1.14 | iodoacetamide | - |
Triticum aestivum | |
| 3.1.1.14 | Mg2+ | - |
Triticum aestivum | |
| 3.1.1.14 | additional information | inhibitory effects on recombinant enzymes purified by microtiter plate method and by affinity chromatography, respectively, overview, no inhibition by iodoacetic acid, iodoacetamide, 2-mercaptoethanol, and DTT | Triticum aestivum | |
| 3.1.1.14 | N-ethylmaleimide | - |
Triticum aestivum | |
| 3.1.1.14 | phenylmethanesulfonyl fluoride | - |
Triticum aestivum | |
| 3.1.1.14 | Zn2+ | - |
Triticum aestivum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.14 | additional information | - |
additional information | steady state kinetics, the recombinant immobilized fusion protein displays kinetic parameters similar to those of recombinant enzyme purified by affnity chromatography, overview | Triticum aestivum | |
| 3.1.1.14 | 0.05 | - |
4-nitrophenyl decanoate | pH 8.0, 25°C, recombinant enzyme purified by affinity chromatography | Triticum aestivum | |
| 3.1.1.14 | 0.069 | - |
4-nitrophenyl decanoate | pH 8.0, 25°C, recombinant enzyme purified by microtiter plate method | Triticum aestivum | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.14 | chlorophyll + H2O | Triticum aestivum | chlorophyllase catalyzes the initial hydrolysis of the phytol moiety from the pigment in the degradation of chlorophyll, overview | phytol + chlorophyllide | - |
? | |
| 3.1.1.14 | additional information | Triticum aestivum | since chlorophyll degradation is a defining feature of plant senescence, compounds inhibiting chlorophyllase activity may delay senescence, thereby improving shelf life and appearance of plant products | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.14 | Triticum aestivum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.14 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, or by using a microtiter plate purification method with immobilization of the enzyme, overview | Triticum aestivum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.14 | additional information | - |
development of a continuous assay system, replacing chlorophyll with p-nitrophenyl-ester substrates eliminates the extraction step and allows for continuous measurement of chlorophyllase activity in the multiwell plate format, overview | Triticum aestivum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.14 | 4-nitrophenyl butyrate + H2O | - |
Triticum aestivum | 4-nitrophenol + butyrate | - |
? | |
| 3.1.1.14 | 4-nitrophenyl decanoate + H2O | - |
Triticum aestivum | 4-nitrophenol + decanoate | - |
? | |
| 3.1.1.14 | chlorophyll + H2O | - |
Triticum aestivum | phytol + chlorophyllide | - |
? | |
| 3.1.1.14 | chlorophyll + H2O | chlorophyllase catalyzes the initial hydrolysis of the phytol moiety from the pigment in the degradation of chlorophyll, overview | Triticum aestivum | phytol + chlorophyllide | - |
? | |
| 3.1.1.14 | additional information | since chlorophyll degradation is a defining feature of plant senescence, compounds inhibiting chlorophyllase activity may delay senescence, thereby improving shelf life and appearance of plant products | Triticum aestivum | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.14 | 25 | - |
assay at | Triticum aestivum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.14 | 8.05 | - |
4-nitrophenyl decanoate | pH 8.0, 25°C, recombinant enzyme purified by affinity chromatography | Triticum aestivum | |
| 3.1.1.14 | 9.43 | - |
4-nitrophenyl decanoate | pH 8.0, 25°C, recombinant enzyme purified by microtiter plate method | Triticum aestivum | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.14 | 7 | - |
assay at, substrate chlorophyll | Triticum aestivum |
| 3.1.1.14 | 8 | - |
assay at, substrate 4-nitrophenyl esters | Triticum aestivum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
