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Literature summary extracted from

  • Gonnety, J.T.; Niamke, S.; Faulet, B.M.; Jean-Parfait, E.; Kouadio, N.; Kouame, L.P.
    Purification and characterization of three low-molecular-weight acid phosphatases from peanut (Arachis hypogaea) seedlings (2006), Afr. J. Biotechnol., 5, 035-044.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.2 dithiothreitol 136.55% activity at 0.1% (w/v) Arachis hypogaea
3.1.3.2 additional information not affected by L-cysteine, tetradecyl-trimethyl-ammoniumbromide 0.1% (w/v) and hexadecyl-trimethyl-ammoniumbromide 0.1% (w/v) Arachis hypogaea
3.1.3.2 Nonidet P 40 107.8% activity at 1% (w/v) Arachis hypogaea
3.1.3.2 Nonidet P 40 146.5% activity at 1% (w/v) Arachis hypogaea
3.1.3.2 Nonidet P 40 166.7% activity at 1% (w/v) Arachis hypogaea
3.1.3.2 polyoxyethylene-9-lauryl ether 120.4% activity at 1% (w/v) Arachis hypogaea
3.1.3.2 polyoxyethylene-9-lauryl ether 125% activity at 1% (w/v) Arachis hypogaea
3.1.3.2 polyoxyethylene-9-lauryl ether 147.4% activity at 1% (w/v) Arachis hypogaea
3.1.3.2 taurocholic acid 114% activity at 1% (w/v) Arachis hypogaea
3.1.3.2 taurocholic acid 136.3% activity at 1% (w/v) Arachis hypogaea
3.1.3.2 taurocholic acid 168.3% activity at 1% (w/v) Arachis hypogaea
3.1.3.2 Triton X-100 124.5% activity at 1% (w/v) Arachis hypogaea
3.1.3.2 Triton X-100 132.5% activity at 1% (w/v) Arachis hypogaea
3.1.3.2 Triton X-100 169.6% activity at 1% (w/v) Arachis hypogaea

General Stability

EC Number General Stability Organism
3.1.3.2 stable in the presence of Na+ and K+ Arachis hypogaea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 5,5'-dithio-2,2' dinitro-dibenzoate 13.26% residual activity at 0.1% (w/v); 39.83% residual activity at 0.1% (w/v); 9.1% residual activity at 0.1% (w/v) Arachis hypogaea
3.1.3.2 Ba2+ 30.3% residual activity at 1 mM; 50.9% residual activity at 1 mM Arachis hypogaea
3.1.3.2 beta-mercaptoethanol 72.72% residual activity at 0.1% (w/v); 75.98% residual activity at 0.1% (w/v); 92.8% residual activity at 0.1% (w/v) Arachis hypogaea
3.1.3.2 Ca2+ 80.2% residual activity at 5 mM; 86.2% residual activity at 5 mM Arachis hypogaea
3.1.3.2 Cu2+ 25.7% residual activity at 5 mM; 30% residual activity at 5 mM; 64.8% residual activity at 1 mM Arachis hypogaea
3.1.3.2 dithiothreitol 64.34% activity at 0.1% (w/v); 98.7% residual activity at 0.1% (w/v) Arachis hypogaea
3.1.3.2 EDTA 40% residual activity at 5 mM; 85% residual activity at 5 mM; 92.2% residual activity at 5 mM Arachis hypogaea
3.1.3.2 Fe3+ 76% residual activity at 5 mM; 85% residual activity at 5 mM Arachis hypogaea
3.1.3.2 hexadecyl-trimethyl-ammoniumbromide 87.8% residual activity at 0.1% (w/v) Arachis hypogaea
3.1.3.2 K+ 92.6% residual activity at 1 mM; 93.4% residual activity at 1 mM Arachis hypogaea
3.1.3.2 L-cysteine 72.72% residual activity at 0.1% (w/v); 85.27% residual activity at 0.1% (w/v) Arachis hypogaea
3.1.3.2 Mg2+ 66.5% residual activity at 5 mM; 90.8% residual activity at 5 mM Arachis hypogaea
3.1.3.2 p-chloromercuribenzoate 62.99% residual activity at 0.1% (w/v); 69.39% residual activity at 0.1% (w/v); 72.81% residual activity at 0.1% (w/v) Arachis hypogaea
3.1.3.2 SDS 10% residual activity at 0.1% (w/v); 22% residual activity at 0.1% (w/v); 3% residual activity at 0.1% (w/v) Arachis hypogaea
3.1.3.2 Sr2+ 45.7% residual activity at 5 mM Arachis hypogaea
3.1.3.2 tetradecyl-trimethyl-ammoniumbromide 50.6% residual activity at 0.1% (w/v) Arachis hypogaea
3.1.3.2 Zn2+ 15% residual activity at 5 mM; 19% residual activity at 5 mM; 3.1% residual activity at 5 mM Arachis hypogaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.38
-
Phenylphosphate at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
3.1.3.2 0.49
-
p-nitrophenyl phosphate at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
3.1.3.2 0.76
-
p-nitrophenyl phosphate at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
3.1.3.2 0.79
-
p-nitrophenyl phosphate at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
3.1.3.2 1.16
-
ATP at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
3.1.3.2 1.31
-
Phenylphosphate at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
3.1.3.2 1.48
-
ADP at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
3.1.3.2 1.5 2 Phenylphosphate at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
3.1.3.2 1.77
-
ATP at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
3.1.3.2 2.88
-
ATP at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
3.1.3.2 4.63
-
ADP at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
3.1.3.2 5.71
-
ADP at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Ba2+ 115% activity at 5 mM Arachis hypogaea
3.1.3.2 Ba2+ 223.3% activity at 5 mM Arachis hypogaea
3.1.3.2 Ca2+ 244% activity at 1 mM Arachis hypogaea
3.1.3.2 Cu2+ 190% activity at 5 mM Arachis hypogaea
3.1.3.2 Fe3+ 186% activity at 1 mM Arachis hypogaea
3.1.3.2 K+ 124.9% activity at 1 mM Arachis hypogaea
3.1.3.2 K+ 127.3% activity at 5 mM Arachis hypogaea
3.1.3.2 Mg2+ 143.6% activity at 5 mM Arachis hypogaea
3.1.3.2 Mg2+ 197% activity at 1 mM Arachis hypogaea
3.1.3.2 Na+ 118% activity at 5 mM Arachis hypogaea
3.1.3.2 Na+ 125% activity at 5 mM Arachis hypogaea
3.1.3.2 Sr2+ 131% activity at 5 mM Arachis hypogaea
3.1.3.2 Sr2+ 193% activity at 5 mM Arachis hypogaea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 12000
-
1 * 14000 + 1 * 12000, isozyme PI, gel filtration Arachis hypogaea
3.1.3.2 14000
-
1 * 14000 + 1 * 12000, isozyme PI, gel filtration Arachis hypogaea
3.1.3.2 22400
-
isozyme PIIa, SDS-PAGE Arachis hypogaea
3.1.3.2 24000
-
isozyme PIIb, SDS-PAGE Arachis hypogaea
3.1.3.2 25300
-
isozyme PI, SDS-PAGE Arachis hypogaea
3.1.3.2 26000
-
isozyme PI, gel filtration Arachis hypogaea

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.3.2 Nonidet P 40 stable in the presence of non-ionic detergents such as Nonidet P 40 Arachis hypogaea
3.1.3.2 Triton X-100 stable in the presence of non-ionic detergents such as Triton X-100 Arachis hypogaea

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Arachis hypogaea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 DEAE-Sepharose CL-6B chromatography, CM-Sepharose CL-6B chromatography, Sephacryl S-200 HR gel filtration, and Phenyl-Sepharose HP column chromatography Arachis hypogaea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 seedling
-
Arachis hypogaea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 0.62
-
crude cell extract Arachis hypogaea
3.1.3.2 30.5
-
isozyme PIIa, after 49.5fold purification Arachis hypogaea
3.1.3.2 36
-
isozyme PIIb, after 58.2fold purification Arachis hypogaea
3.1.3.2 54.7
-
isozyme PI, after 92fold purification Arachis hypogaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 adenosine-2',3'-cyclomonophosphate + H2O 63% activity compared to p-nitrophenyl phosphate Arachis hypogaea ?
-
?
3.1.3.2 adenosine-2',3'-cyclomonophosphate + H2O 9% activity compared to p-nitrophenyl phosphate Arachis hypogaea ?
-
?
3.1.3.2 ADP + H2O 100.2% activity compared to p-nitrophenyl phosphate Arachis hypogaea AMP + phosphate
-
?
3.1.3.2 ADP + H2O 103.4% activity compared to p-nitrophenyl phosphate Arachis hypogaea AMP + phosphate
-
?
3.1.3.2 ADP + H2O 81.5% activity compared to p-nitrophenyl phosphate Arachis hypogaea AMP + phosphate
-
?
3.1.3.2 AMP + H2O 25.2% activity compared to p-nitrophenyl phosphate Arachis hypogaea adenosine + phosphate
-
?
3.1.3.2 AMP + H2O 25.5% activity compared to p-nitrophenyl phosphate Arachis hypogaea adenosine + phosphate
-
?
3.1.3.2 AMP + H2O 51.5% activity compared to p-nitrophenyl phosphate Arachis hypogaea adenosine + phosphate
-
?
3.1.3.2 ATP + H2O 116.7% activity compared to p-nitrophenyl phosphate Arachis hypogaea ADP + phosphate
-
?
3.1.3.2 ATP + H2O 76.9% activity compared to p-nitrophenyl phosphate Arachis hypogaea ADP + phosphate
-
?
3.1.3.2 ATP + H2O 80.7% activity compared to p-nitrophenyl phosphate Arachis hypogaea ADP + phosphate
-
?
3.1.3.2 D-galactose 1-phosphate + H2O 44.7% activity compared to p-nitrophenyl phosphate Arachis hypogaea D-galactose + phosphate
-
?
3.1.3.2 D-galactose 1-phosphate + H2O 8.8% activity compared to p-nitrophenyl phosphate Arachis hypogaea D-galactose + phosphate
-
?
3.1.3.2 D-glucose 1-phosphate + H2O 21.4% activity compared to p-nitrophenyl phosphate Arachis hypogaea D-glucose + phosphate
-
?
3.1.3.2 D-glucose 6-phosphate + H2O 30.4% activity compared to p-nitrophenyl phosphate Arachis hypogaea D-glucose + phosphate
-
?
3.1.3.2 diphosphate + H2O 56.1% activity compared to p-nitrophenyl phosphate Arachis hypogaea 2 phosphate
-
?
3.1.3.2 diphosphate + H2O 66.35% activity compared to p-nitrophenyl phosphate Arachis hypogaea 2 phosphate
-
?
3.1.3.2 additional information no activity with adenosine-2',3'-cyclomonophosphate, D-glucose 1-phosphate, and D-glucose 6-phosphate Arachis hypogaea ?
-
?
3.1.3.2 additional information no activity with D-glucose 1-phosphate, D-glucose 6-phosphate, and sodium phytate Arachis hypogaea ?
-
?
3.1.3.2 additional information no activity with uridyl-5'-phosphogalactose, D-galactose 1-phosphate, and sodium phytate Arachis hypogaea ?
-
?
3.1.3.2 NAD+ + H2O 15.4% activity compared to p-nitrophenyl phosphate Arachis hypogaea ?
-
?
3.1.3.2 NAD+ + H2O 16.5% activity compared to p-nitrophenyl phosphate Arachis hypogaea ?
-
?
3.1.3.2 NAD+ + H2O 26.6% activity compared to p-nitrophenyl phosphate Arachis hypogaea ?
-
?
3.1.3.2 NADP+ + H2O 28% activity compared to p-nitrophenyl phosphate Arachis hypogaea NAD+ + phosphate
-
?
3.1.3.2 NADP+ + H2O 36% activity compared to p-nitrophenyl phosphate Arachis hypogaea NAD+ + phosphate
-
?
3.1.3.2 NADP+ + H2O 61.7% activity compared to p-nitrophenyl phosphate Arachis hypogaea NAD+ + phosphate
-
?
3.1.3.2 p-nitrophenyl phosphate + H2O 100% activity Arachis hypogaea p-nitrophenol + phosphate
-
?
3.1.3.2 phenylphosphate + H2O 85.2% activity compared to p-nitrophenyl phosphate Arachis hypogaea phenol + phosphate
-
?
3.1.3.2 phenylphosphate + H2O 89% activity compared to p-nitrophenyl phosphate Arachis hypogaea phenol + phosphate
-
?
3.1.3.2 phenylphosphate + H2O 93.1% activity compared to p-nitrophenyl phosphate Arachis hypogaea phenol + phosphate
-
?
3.1.3.2 phytate + H2O 20.1% activity compared to p-nitrophenyl phosphate Arachis hypogaea ?
-
?
3.1.3.2 sodium diphosphate + H2O 87% activity compared to p-nitrophenyl phosphate Arachis hypogaea sodium phosphate + phosphate
-
?
3.1.3.2 uridyl-5'-phosphogalactose + H2O 21% activity compared to p-nitrophenyl phosphate Arachis hypogaea uridyl-5'-galactose + phosphate
-
?
3.1.3.2 uridyl-5'-phosphogalactose + H2O 25.5% activity compared to p-nitrophenyl phosphate Arachis hypogaea uridyl-5'-galactose + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 heterodimer 1 * 14000 + 1 * 12000, isozyme PI, gel filtration Arachis hypogaea
3.1.3.2 monomer 1 * 22400, isozyme PIIa, SDS-PAGE Arachis hypogaea
3.1.3.2 monomer 1 * 24000, isozyme PIIb, SDS-PAGE Arachis hypogaea

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 acid phosphatase PI isozyme Arachis hypogaea
3.1.3.2 acid phosphatase PIIa isozyme, exhibits phytasic activity Arachis hypogaea
3.1.3.2 acid phosphatase PIIb isozyme Arachis hypogaea
3.1.3.2 orthophosphoric-monoester phosphohydrolase
-
Arachis hypogaea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 55
-
-
Arachis hypogaea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 55 80 above 55°C, the activity declines rapidly as the temperature increases, but the enzyme is not completely inactivated even at 80°C Arachis hypogaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5
-
-
Arachis hypogaea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.2 4.6 5.6 at 37°C Arachis hypogaea
3.1.3.2 5 5.6 at 37°C Arachis hypogaea