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Literature summary extracted from

  • Ulaganathan, V.; Agacan, M.F.; Buetow, L.; Tulloch, L.B.; Hunter, W.N.
    Structure of Staphylococcus aureus1,4-dihydroxy-2-naphthoyl-CoA synthase (MenB) in complex with acetoacetyl-CoA (2007), Acta Crystallogr. Sect. F, 63, 908-913.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.36 expressed in Escherichia coli strain BL21(DE3) Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.3.36 sitting drop vapour diffusion method with 0.1 M NaHEPES pH 7.5, 1.6 M ammonium sulfate, 0.2 M NaCl Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.36 185000
-
 gel filtration Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.36 Staphylococcus aureus Q5HH38
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.36 HiTrap Chelating HP column chromatography and HiTrap column chromatography Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.36 o-succinylbenzoyl-CoA
-
 Staphylococcus aureus 1,4-dihydroxy-2-naphthoyl-CoA + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.36 hexamer gel filtration Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
4.1.3.36 1,4-dihydroxy-2-naphthoyl-CoA synthase
-
 Staphylococcus aureus
4.1.3.36 MenB
-
 Staphylococcus aureus