EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.4.2 | expression in Escherichia coli | Klebsiella aerogenes |
3.1.4.46 | expressed in Escherichia coli DH5alpha | Klebsiella aerogenes |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.4.2 | in complex with Zn and Co as well as without metals in apo-form | Klebsiella aerogenes |
3.1.4.46 | sitting drop vapor diffusion method, best crystallization results when apo-GpdQ is used, crystals with bound Zn2+ and Co2+ | Klebsiella aerogenes |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.4.2 | 30840 | - |
calculated from amino acid-sequence | Klebsiella aerogenes |
3.1.4.2 | 31000 | - |
determined by SDS-PAGE | Klebsiella aerogenes |
3.1.4.46 | 31000 | - |
2 * 31000, SDS-PAGE, crystal structure analysis | Klebsiella aerogenes |
3.1.4.46 | 3083993 | - |
2 * 3083993, calculated from the deduced amino acid sequence, crystal structure analysis | Klebsiella aerogenes |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.46 | glycerophosphocholine + H2O | Klebsiella aerogenes | maintenance of the sn-glycerol-3-phosphate concentration which is essential to enable phospholipid synthesis | sn-glycerol 3-phosphate + choline | - |
? | |
3.1.4.46 | glycerophosphodiester + H2O | Klebsiella aerogenes | maintenance of the sn-glycerol-3-phosphate concentration which is essential to enable phospholipid synthesis | sn-glycerol 3-phosphate + alcohol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.2 | Klebsiella aerogenes | - |
- |
- |
3.1.4.46 | Klebsiella aerogenes | Q6XBH1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.4.2 | - |
Klebsiella aerogenes |
3.1.4.46 | recombinant protein | Klebsiella aerogenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.2 | glycerophosphocholine + H2O | - |
Klebsiella aerogenes | sn-glycerol 3-phosphate + choline | - |
? | |
3.1.4.46 | glycerophosphocholine + H2O | maintenance of the sn-glycerol-3-phosphate concentration which is essential to enable phospholipid synthesis | Klebsiella aerogenes | sn-glycerol 3-phosphate + choline | - |
? | |
3.1.4.46 | glycerophosphodiester + H2O | maintenance of the sn-glycerol-3-phosphate concentration which is essential to enable phospholipid synthesis | Klebsiella aerogenes | sn-glycerol 3-phosphate + alcohol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.4.2 | dimer | homodimer | Klebsiella aerogenes |
3.1.4.46 | ? | data also indicate the possibility of a tetrameric organization or other number of monomers | Klebsiella aerogenes |
3.1.4.46 | dimer | 2 * 3083993, calculated from the deduced amino acid sequence, crystal structure analysis | Klebsiella aerogenes |
3.1.4.46 | dimer | 2 * 31000, SDS-PAGE, crystal structure analysis | Klebsiella aerogenes |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.2 | glycerophosphodiesterase | - |
Klebsiella aerogenes |
3.1.4.2 | GpdQ | - |
Klebsiella aerogenes |
3.1.4.2 | metallo-glycerophosphodiesterase | - |
Klebsiella aerogenes |
3.1.4.46 | GpdQ | - |
Klebsiella aerogenes |
3.1.4.46 | metallo-glycerophosphodiesterase | - |
Klebsiella aerogenes |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.4.2 | Klebsiella aerogenes | theoretical value | - |
5.45 |