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Literature summary extracted from

  • Cui, Z.; Maruyama, Y.; Mikami, B.; Hashimoto, W.; Murata, K.
    Crystallization and preliminary crystallographic analysis of the family GH78 alpha-L-rhamnosidase RhaB from Bacillus sp. GL1 (2006), Acta Crystallogr. Sect. F, 62, 646-648.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.40 expressed in Escherichia coli B834-DE3 containing pET3a-RhaB plasmid Bacillus sp. (in: Bacteria)

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.40 native and selenomethionine-derivaties, crystallized at 293 K, hanging-drop vapour diffusion with PEG 8000 as a precipitant, data collection and statistics indicated Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.40 gellan + H2O Bacillus sp. (in: Bacteria) hydrolytic reaction, release of rhamnose from the disaccharide alpha-L-rhamnose + ?
-
 ?
3.2.1.40 gellan + H2O Bacillus sp. (in: Bacteria) GL1 hydrolytic reaction, release of rhamnose from the disaccharide alpha-L-rhamnose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Bacillus sp. (in: Bacteria) Q93RE7 rhamnosidase B, RhaB
-
3.2.1.40 Bacillus sp. (in: Bacteria) GL1 Q93RE7 rhamnosidase B, RhaB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40 recombinant protein, gel filtration, SDS-PAGE Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.40 additional information
-
 assay in a reaction system containing 0.4 mM p-nitrophenyl-alpha-L-rhamnopyranoside as a substrate and 50 mM potassium phosphate buffer, resultant p-nitrophenol concentration determined spectrophotometrically Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 4-nitrophenyl-alpha-L-rhamnopyranoside + H2O
-
 Bacillus sp. (in: Bacteria) 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 4-nitrophenyl-alpha-L-rhamnopyranoside + H2O
-
 Bacillus sp. (in: Bacteria) GL1 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 gellan + H2O hydrolytic reaction, release of rhamnose from the disaccharide Bacillus sp. (in: Bacteria) alpha-L-rhamnose + ?
-
 ?
3.2.1.40 gellan + H2O release of rhamnose from the substrate, hydrolytic reaction Bacillus sp. (in: Bacteria) alpha-L-rhamnose + ?
-
 ?
3.2.1.40 gellan + H2O hydrolytic reaction, release of rhamnose from the disaccharide Bacillus sp. (in: Bacteria) GL1 alpha-L-rhamnose + ?
-
 ?
3.2.1.40 gellan + H2O release of rhamnose from the substrate, hydrolytic reaction Bacillus sp. (in: Bacteria) GL1 alpha-L-rhamnose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.40 alpha-L-rhamnosidase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.40 30
-
 assay at Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 7
-
 assay at Bacillus sp. (in: Bacteria)