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Literature summary extracted from

  • Yoshida, H.; Wayoon, P.; Takada, G.; Izumori, K.; Kamitori, S.
    Crystallization and preliminary X-ray diffraction studies of L-rhamnose isomerase from Pseudomonas stutzeri (2006), Acta Crystallogr. Sect. F, 62, 550-552.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.14 expression of His6-tagged enzyme in Escherichia coli Pseudomonas stutzeri

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.14 purified recombinant His-tagged L-RhI by hanging-drop vapour-diffusion method, 0.002 ml of 20 mg/ml protein in 20 mM Tris-HCl, pH 8.0, is mixed with 0.002 ml reservcoir solution containing 7-8% w/v PEG 20 000 and 50 mM MES buffer, pH 6.3, equilibration against 0.5 ml reservoir solution, X-ray diffraction structure determination and analysis at 2.0 A resolution Pseudomonas stutzeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.14 43000
-
 x * 43000, SDS-PAGE, x * 47681, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry Pseudomonas stutzeri
5.3.1.14 47681
-
 x * 43000, SDS-PAGE, x * 47681, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.14 L-Rhamnose Pseudomonas stutzeri
-
 L-Rhamnulose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.14 Pseudomonas stutzeri Q75WH8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.14 recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and dialysis Pseudomonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.14 L-Rhamnose
-
 Pseudomonas stutzeri L-Rhamnulose
-
 r
5.3.1.14 L-Rhamnose L-RhI catalyzes not only the reversible isomerization of L-rhamnose to L-rhamnulose, but also isomerization between various rare aldoses and ketoses Pseudomonas stutzeri L-Rhamnulose
-
 r
5.3.1.14 additional information the Pseudomonas stutzeri enzyme shows a broader substrate specificity compared to the enzyme from Escherichia coli, overview Pseudomonas stutzeri ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.14 ? x * 43000, SDS-PAGE, x * 47681, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry Pseudomonas stutzeri

Synonyms

EC Number Synonyms Comment Organism
5.3.1.14 L-rhamnose isomerase
-
 Pseudomonas stutzeri
5.3.1.14 L-RhI
-
 Pseudomonas stutzeri