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Literature summary extracted from

  • Ou, Z.; Felts, R.L.; Reilly, T.J.; Nix, J.C.; Tanner, J.J.
    Crystallization of recombinant Haemophilus influenzae e (P4) acid phosphatase (2006), Acta Crystallogr. Sect. F, 62, 464-466.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.2 expressed in Escherichia coli strain BL21(DE3) Haemophilus influenzae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.2 sitting drop vapour diffusion method with of 0.2 M MgCl2, 0.1 M Tris-HCl pH 8.1-8.5 and 28-36% (w/v) PEG 4000 Haemophilus influenzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 28510
-
 full-length recombinant P4, mass spectrometry analysis Haemophilus influenzae

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Haemophilus influenzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 HiTrap Chelating HP column chromatography, Cu-affinity column chromatography, and HiTrap SP Sepharose column chromatography Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 phosphoenolpyruvate + H2O
-
 Haemophilus influenzae pyruvate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 lipoprotein e class C acid phosphatase Haemophilus influenzae
3.1.3.2 P4
-
 Haemophilus influenzae