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Literature summary extracted from

  • Li, X.; Huang, H.; Song, X.; Wang, Y.; Xu, H.; Teng, M.; Gong, W.
    Purification, crystallization and preliminary crystallographic studies on 2-dehydro-3-deoxygalactarate aldolase from Leptospira interrogans (2006), Acta Crystallogr. Sect. F, 62, 1269-1270.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.20 expressed in Escherichia coli BL21 (DE3) cells Leptospira interrogans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.20 hanging drop vapour diffusion method with 10% PEG 8000, 100 mM Tris-HCl buffer pH 6.8 and 20 mM MnCl2 Leptospira interrogans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.20 Mg2+ required Leptospira interrogans
4.1.2.20 Mn2+ required Leptospira interrogans

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.20 Leptospira interrogans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.20 DEAE-Sepharose column chromatography and Superdex 75 gel filtration Leptospira interrogans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.20 2-dehydro-3-deoxy-D-glucarate
-
 Leptospira interrogans pyruvate + tartronate semialdehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.20 2-dehydro-3-deoxygalactarate aldolase
-
 Leptospira interrogans
4.1.2.20 DDG aldolase
-
 Leptospira interrogans