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Literature summary extracted from

  • Carr, S.B.; Makris, G.; Phillips, S.E.; Thomas, C.D.
    Crystallization and preliminary X-ray diffraction analysis of two N-terminal fragments of the DNA-cleavage domain of topoisomerase IV from Staphylococcus aureus (2006), Acta Crystallogr. Sect. F, 62, 1164-1167.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
5.6.2.2 pharmacology Topo II are attractive targets for design of antitumor agents Staphylococcus aureus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.2.2 Protein expression performed in Escherichia coli B834 DE3 (plysS) Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.6.2.2 first report of crystallization and preliminary X-ray analysis of the DNA-cleavage domain of a topoisomerase IV from a gram positive organism. Sparse-matrix screening used, Crystals of both protein fragments (GrlA56 and GrlA59) display a plate-like morphology and initially grow in tightly packed clusters. Crystallization and X-ray analysis of two different constructs of the A subunit of topoisomerase IV from Staphylococcus aureus (GrlA) are described. With a view to gaining further insight into the mode of inhibition of quinolone antibiotics against this enzyme Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.2.2 56000
-
 generated protein GrlA56 Staphylococcus aureus
5.6.2.2 59000
-
 generated protein GrlA59 Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.2 Staphylococcus aureus Q6G9K4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.2
-
 Staphylococcus aureus

Subunits

EC Number Subunits Comment Organism
5.6.2.2 heterotetramer Two constructs of 56 and 59 kDa spanning the DNA-cleavage domain of the A subunit of topoisomerase IV from Staphylococcus aureus. All bacterial type IIA topoisomerases are A2B2 heterotetramers containing three discrete subunit interfaces which open and close sequentially in response to ATP binding and hydrolysis in order to effect DNA transport Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
5.6.2.2 DNA topoisomerase IV type IIA DNA topoisomerase Staphylococcus aureus
5.6.2.2 topoIV
-
 Staphylococcus aureus