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Literature summary extracted from

  • Oganesyan, V.; Huang, C.; Adams, P.D.; Jancarik, J.; Yokota, H.A.; Kim, R.; Kim, S.H.
    Structure of a NAD kinase from Thermotoga maritima at 2.3 A resolution (2005), Acta Crystallogr. Sect. F, 61, 640-646.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.23 expression in Escherichia coli Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.23 crystals of SeMet-containing PPNK_THEMA are obtained at room temperature in hanging drops. Crystal structure of inorganic polyphosphate/ATP-NAD kinase is determined at 2.3 A resolution. The crystal structure is solved using single-wavelength anomalous diffraction data collected at the Se absorption-peak wavelength in a state in which no cofactors or substrates are bound. It revealed that the 258-amino-acid protein is folded into two distinct domains. The N-terminal alpha/beta-domain spans the first 100 amino acids and the last 30 amino acids of the polypeptide and has several topological matches in the PDB, whereas the other domain, which spans the middle 130 residues, adopts a unique beta-sandwich architecture and only appreciably matches the recently deposited PDB structures of NAD kinases Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.23 ATP + NAD+ Thermotoga maritima
-
 ADP + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.23 Thermotoga maritima Q9X255
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.7.1.23 recombinant enzyme Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.23 ATP + NAD+
-
 Thermotoga maritima ADP + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.23 PPNK_THEMA
-
 Thermotoga maritima