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Literature summary extracted from

  • Leonard, P.M.; Brzozowski, A.M.; Lebedev, A.; Marshall, C.M.; Smith, D.J.; Verma, C.S.; Walton, N.J.; Grogan, G.
    The 1.8 A resolution structure of hydroxycinnamoyl-coenzyme A hydratase-lyase (HCHL) from Pseudomonas fluorescens, an enzyme that catalyses the transformation of feruloyl-coenzyme A to vanillin (2006), Acta Crystallogr. Sect. D, 62, 1494-1501.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.2.61 additional information modelling data as a prerequisite for enzyme engineering Pseudomonas fluorescens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.61 expressed in Escherichia coli strain BL21 to yield HCHL protein encoded by Pseudomonas fluorescens strain AN103 Pseudomonas fluorescens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.61 1.8 A resolution, data collection and refinement statistics shown, structure solution and in silico modelling determined, monomer folding and factors affecting trimer assembly indicated, oligo structure and modelling of the active site determined Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.61 feruloyl-CoA Pseudomonas fluorescens
-
 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.61 Pseudomonas fluorescens
-
 Pseudomonas fluorescens AN103
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.61 feruloyl-CoA
-
 Pseudomonas fluorescens 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 feruloyl-CoA modelling of the natural substrate feruloyl-CoA in the active site of HCHL reveals a catalytic role for Glu143 and a substrate-specific evidence for Tyr239 from the adjacent monomeric structure Pseudomonas fluorescens 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA catalyzes the hydration of the acyl-CoA thioester of ferulic acid, hydration of the double bond yields 4-hydroxy-3-methoxy-phenyl-beta-hydroxy-propionyl-CoA, subsequent retro-aldol cleavage leads to vanilin ?

Subunits

EC Number Subunits Comment Organism
4.1.2.61 More hexameric structure of two stacked trimers assumed, similarities to the crotonase superfamily Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
4.1.2.61 HCHL
-
 Pseudomonas fluorescens
4.1.2.61 hydroxycinnamoyl-CoA hydratase/lyase
-
 Pseudomonas fluorescens