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Literature summary extracted from

  • Rahman, F.B.; Yamauchi, K.
    Uncompetitive inhibition of Xenopus laevis aldehyde dehydrogenase 1A1 by divalent cations (2006), Zool. Sci., 23, 239-244.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.36 expression in Escherichia coli BL21 (DE3) as His-tag fusion protein Xenopus laevis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.36 Ca2+ 50% inhibition at 0.22 mM Xenopus laevis
1.2.1.36 Mg2+ 50% inhibition at 0.035 mM Xenopus laevis
1.2.1.36 Mn2+ 50% inhibition at 0.024 mM Xenopus laevis
1.2.1.36 Zn2+ 50% inhibition at 0.01 mM Xenopus laevis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.36 0.001
-
 retinal 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.03 mM retinal, 0.056-0.12 mM NAD+ Xenopus laevis
1.2.1.36 0.0044
-
 retinal 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.5 mM Ca2+, 0.03 mM retinal, 0.056-0.12 mM NAD+ Xenopus laevis
1.2.1.36 0.0046
-
 retinal 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.04 mM Mn2+, 0.03 mM retinal, 0.056-0.12 mM NAD+ Xenopus laevis
1.2.1.36 0.006
-
 retinal 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.3 mM Mg2+, 0.03 mM retinal, 0.056-0.12 mM NAD+ Xenopus laevis
1.2.1.36 0.0064
-
 retinal 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.03 mM Zn2+ 0.03 mM retinal, 0.056-0.12 mM NAD+ Xenopus laevis
1.2.1.36 0.019
-
 acetaldehyde 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.01 mM Zn2+, 12 mM acetaldehyde, 0.015-0.024 mM NAD+ Xenopus laevis
1.2.1.36 0.024
-
 acetaldehyde 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.02 Mn2+, 12 mM acetaldehyde, 0.015-0.024 mM NAD+ Xenopus laevis
1.2.1.36 0.027
-
 acetaldehyde 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.03 mM Ca2+, 12 mM acetaldehyde, 0.015-0.024 mM NAD+ Xenopus laevis
1.2.1.36 0.028
-
 acetaldehyde 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.1 mM Mg2+, 12 mM acetaldehyde, 0.015-0.024 mM NAD+ Xenopus laevis
1.2.1.36 0.055
-
 acetaldehyde 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 12 mM acetaldehyde, 0.015-0.024 mM NAD+ Xenopus laevis
1.2.1.36 1.4
-
 acetaldehyde 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.3 mM Ca2+, 1-12 mM acetaldehyde, 0.33 mM NAD+ Xenopus laevis
1.2.1.36 1.8
-
 acetaldehyde 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.1 mM Mg2+, 1-12 mM acetaldehyde, 0.33 mM NAD+ Xenopus laevis
1.2.1.36 2
-
 acetaldehyde 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.02 mM Mn2+, 1-12 mM acetaldehyde, 0.33 mM NAD+ Xenopus laevis
1.2.1.36 2.1
-
 acetaldehyde 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.01 mM Zn2+, 1-12 mM acetaldehyde, 0.33 mM NAD+ Xenopus laevis
1.2.1.36 3.4
-
 acetaldehyde 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 1-12 mM acetaldehyde, 0.33 mM NAD+ Xenopus laevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.36 58000
-
 SDS-PAGE, recombinant protein Xenopus laevis

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.36 Xenopus laevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.36 Ni-affinity chromatography Xenopus laevis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.36 cell culture
-
 Xenopus laevis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.36 acetaldehyde + NAD+ + H2O
-
 Xenopus laevis acetate + NADH + H+
-
 ?
1.2.1.36 retinal + NAD+ + H2O
-
 Xenopus laevis retinoic acid + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.36 aldehyde dehydrogenase 1A1
-
 Xenopus laevis
1.2.1.36 ALDH class 1
-
 Xenopus laevis
1.2.1.36 ALDH1
-
 Xenopus laevis
1.2.1.36 ALDH1A1
-
 Xenopus laevis
1.2.1.36 RalDH1
-
 Xenopus laevis
1.2.1.36 retinaldehyde dehydrogenase
-
 Xenopus laevis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.36 NAD+
-
 Xenopus laevis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.36 0.005
-
 Zn2+ 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 12 mM acetaldehyde, 0.015-0.024 mM NAD+ Xenopus laevis
1.2.1.36 0.011
-
 Zn2+ 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 1-12 mM acetaldehyde, 0.33 mM NAD+ Xenopus laevis
1.2.1.36 0.015
-
 Mn2+ 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 12 mM acetaldehyde, 0.015-0.024 mM NAD+ Xenopus laevis
1.2.1.36 0.036
-
 Zn2+ 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.03 mM retinal, 0.056-0.12 mM NAD+ Xenopus laevis
1.2.1.36 0.04
-
 Mn2+ 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 1-12 mM acetaldehyde, 0.33 mM NAD+ Xenopus laevis
1.2.1.36 0.052
-
 Mn2+ 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.03 mM retinal, 0.056-0.12 mM NAD+ Xenopus laevis
1.2.1.36 0.11
-
 Mg2+ 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 12 mM acetaldehyde, 0.015-0.024 mM NAD+ Xenopus laevis
1.2.1.36 0.17
-
 Mg2+ 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 1-12 mM acetaldehyde, 0.33 mM NAD+ Xenopus laevis
1.2.1.36 0.27
-
 Ca2+ 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 12 mM acetaldehyde, 0.015-0.024 mM NAD+ Xenopus laevis
1.2.1.36 0.3
-
 Ca2+ 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 1-12 mM acetaldehyde, 0.33 mM NAD+ Xenopus laevis
1.2.1.36 0.349
-
 Mg2+ 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.03 mM retinal, 0.056-0.12 mM NAD+ Xenopus laevis
1.2.1.36 0.479
-
 Ca2+ 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.03 mM retinal, 0.056-0.12 mM NAD+ Xenopus laevis