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Literature summary extracted from

  • Orlowski, J.; Machula, K.; Janik, A.; Zdebska, E.; Palamarczyk, G.
    Dissecting the role of dolichol in cell wall assembly in the yeast mutants impaired in early glycosylation reactions (2007), Yeast, 24, 239-252.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.83 additional information DPM synthase mutants have an aberrant cell wall composition and ultrastructure. Mutant is oversensitive to Calcofluor white, an agent interacting with the cell wall chitin. Chemical analysis of the cell wall alkali-insoluble fraction indicates an increased amount of chitin and changes in the quantity of beta1,6- and beta1,3-glucan in dpm1-6 mutants. Cell wall defect is suppressed by an increased dolichol availability caused by the RER2 (cis-prenyl transferase-encoding) gene overexpression and subsequent upregulation of DolPPGlcNAc2 synthesis. This is concomitant with an increase in glycosylation and stability of the plasma membrane protein Gas1 and rearrangement of the cell wall components Saccharomyces cerevisiae
2.7.1.108 W332G mutated Sec59-1p bears the single amino acid substitution Trp(332) to Gly, region predicted to be responsible for the substrate (dolichol) binding Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.83 Saccharomyces cerevisiae
-
-
-
2.7.1.108 Saccharomyces cerevisiae
-
 temperature-sensitive Saccharomyces cerevisiae mutants, impaired in dolichol kinase (Sec59p) or dolichyl phosphate mannose synthase (Dpm1p) activity have an aberrant cell wall composition and ultrastructure
-
2.7.1.108 Saccharomyces cerevisiae
-
 (Ferro-Novick et al., 1984), temperature sensitive mutants, mutants impaired in Sec59p (sec59-1), cells are defective in CTP-dependent dolichol kinase
-
2.7.1.108 Saccharomyces cerevisiae SF402-4D
-
 (Ferro-Novick et al., 1984), temperature sensitive mutants, mutants impaired in Sec59p (sec59-1), cells are defective in CTP-dependent dolichol kinase
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.108 cell culture temperature-sensitive Saccharomyces cerevisiae mutants, impaired in dolichol kinase (Sec59p) or dolichyl phosphate mannose synthase (Dpm1p) activity have an aberrant cell wall composition and ultrastructure Saccharomyces cerevisiae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.108 CTP + dolichol
-
 Saccharomyces cerevisiae CDP + dolichyl phosphate
-
 ?
2.7.1.108 CTP + dolichol
-
 Saccharomyces cerevisiae SF402-4D CDP + dolichyl phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.83 dolichol phosphoryl mannose synthase
-
 Saccharomyces cerevisiae
2.4.1.83 DPM
-
 Saccharomyces cerevisiae
2.7.1.108 cytidine-5-triphosphate (CTP) dependent dolichol kinase
-
 Saccharomyces cerevisiae
2.7.1.108 Sec59p
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.108 CTP cytidine-5'-triphosphate Saccharomyces cerevisiae