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Literature summary extracted from

  • Garofano, A.; Eschemann, A.; Brandt, U.; Kerscher, S.
    Substrate-inducible versions of internal alternative NADH: ubiquinone oxidoreductase from Yarrowia lipolytica (2006), Yeast, 23, 1129-1136.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.5.9 expressed in Escherichia coli BL21(DE3) pLysS cells Yarrowia lipolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.9 additional information the enzyme is rotenone-insensitive Yarrowia lipolytica
7.1.1.2 2-decyl-4-quinazolinyl amine 0.002 mM, strong inhibitor Yarrowia lipolytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.5.9 mitochondrion alternative NADH dehydrogenase activity is located exclusively at the external face of the mitochondrial inner membrane Yarrowia lipolytica 5739
-
7.1.1.2 mitochondrial membrane alternative NADH dehydrogenase activity is located exclusively at the external face of the mitochondrial inner membrane Yarrowia lipolytica 31966
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.9 55000
-
 isoform NDH2i, SDS-PAGE Yarrowia lipolytica
1.6.5.9 60000
-
 isoform NDH2e, SDS-PAGE Yarrowia lipolytica

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.9 Yarrowia lipolytica
-
-
-
7.1.1.2 Yarrowia lipolytica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.2 Sepharose Fast Flow column chromatography Yarrowia lipolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.9 additional information NDH2 does not translocate protons across the inner mitochondrial membrane Yarrowia lipolytica ?
-
 ?
1.6.5.9 NADH + H+ + n-decylubiquinone
-
 Yarrowia lipolytica NAD+ + n-decylubiquinol
-
 ?
7.1.1.2 NADH + n-decylubiquinone + H+
-
 Yarrowia lipolytica NAD+ + n-decylubiquinol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.6.5.9 alternative NADH dehydrogenase
-
 Yarrowia lipolytica
1.6.5.9 external alternative NADH dehydrogenase
-
 Yarrowia lipolytica
1.6.5.9 external alternative NADH: ubiquinone oxidoreductase
-
 Yarrowia lipolytica
1.6.5.9 internal alternative NADH dehydrogenase
-
 Yarrowia lipolytica
1.6.5.9 internal alternative NADH: ubiquinone oxidoreductase
-
 Yarrowia lipolytica
1.6.5.9 NDH2e isoform Yarrowia lipolytica
1.6.5.9 NDH2i isoform Yarrowia lipolytica
7.1.1.2 alternative NADH: ubiquinone oxidoreductase
-
 Yarrowia lipolytica
7.1.1.2 NADH: n-decylubiquinone oxidoreductase
-
 Yarrowia lipolytica
7.1.1.2 NADH:DBQ oxidoreductase
-
 Yarrowia lipolytica
7.1.1.2 proton-translocating NADH: ubiquinone oxidoreductase
-
 Yarrowia lipolytica
7.1.1.2 respiratory chain complex I
-
 Yarrowia lipolytica

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.9 FAD
-
 Yarrowia lipolytica
1.6.5.9 NADH
-
 Yarrowia lipolytica
7.1.1.2 NADH
-
 Yarrowia lipolytica

General Information

EC Number General Information Comment Organism
1.6.5.9 physiological function isoform NDH2i allows survival of double mutants lacking both complex I and isoform NDH2e Yarrowia lipolytica