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Literature summary extracted from

  • Wang, Y.; Zheng, Y.; Xue, J.; Shen, Y.
    Characterization of nitrile hydratation catalyzed by Nocardia sp. 108 (2007), World J. Microbiol. Biotechnol., 23, 355-362.
No PubMed abstract available

Application

EC Number Application Comment Organism
4.2.1.84 pharmacology synthesis, biotransformation and biocatalysis of unsaturated/saturated aliphatic, aromatic and heterocyclic nitriles Nocardia sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.84 Co2+ no enzyme activity in medium without cobalt Nocardia sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Nocardia sp.
-
-
-
4.2.1.84 Nocardia sp. 108
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 3,4,5-trimethoxybenzonitrile + H2O conversion rate: 21.71% Nocardia sp. 3,4,5-trimethoxybenzamide
-
 ?
4.2.1.84 3,4,5-trimethoxybenzonitrile + H2O conversion rate: 21.71% Nocardia sp. 108 3,4,5-trimethoxybenzamide
-
 ?
4.2.1.84 3-cyanopyridine + H2O
-
 Nocardia sp. pyridine-3-carboxamide
-
 ?
4.2.1.84 3-cyanopyridine + H2O
-
 Nocardia sp. 108 pyridine-3-carboxamide
-
 ?
4.2.1.84 acrylonitrile + H2O
-
 Nocardia sp. acrylamide
-
 ?
4.2.1.84 acrylonitrile + H2O
-
 Nocardia sp. 108 acrylamide
-
 ?
4.2.1.84 indole-3-acetonitrile + H2O conversion rate: 34.44% Nocardia sp. indole-3-acetamide
-
 ?
4.2.1.84 indole-3-acetonitrile + H2O conversion rate: 34.44% Nocardia sp. 108 indole-3-acetamide
-
 ?
4.2.1.84 o-chlorobenzonitrile + H2O
-
 Nocardia sp. o-chlorobenzamide
-
 ?
4.2.1.84 o-chlorobenzonitrile + H2O
-
 Nocardia sp. 108 o-chlorobenzamide
-
 ?
4.2.1.84 p-aminobenzonitrile + H2O conversion rate: 8.98% Nocardia sp. p-aminobenzamide
-
 ?
4.2.1.84 p-chlorobenzonitrile + H2O conversion rate: 93.1% Nocardia sp. p-chlorobenzamide
-
 ?
4.2.1.84 p-hydroxybenzylcyanide + H2O
-
 Nocardia sp. 2-(4-hydroxyphenyl)acetamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.84 nitrile hydratase
-
 Nocardia sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.84 28
-
-
 Nocardia sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.84 15 28 10°C: 510.5 U/g DCW (dry cell weight), 28°C: 1209.8 U/g DCW (dry cell weight), 30°C: 1155.8 U/g DCW (dry cell weight), 40°C: 677.0 U/g DCW (dry cell weight) Nocardia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.84 7
-
 hydration of p-hydroxybenzylcyanide, 3,4,5-trimethoxybenzonitrile, p-aminobenzonitrile and o-chlorobenzonitrile Nocardia sp.
4.2.1.84 7.5
-
 hydration of acrylonitrile, indole-3-acetonitrile, p-chlorobenzonitrile and 3-cyanopyridine Nocardia sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.84 additional information
-
 different buffer systems including phthalate, phosphate and borax buffer are tested for influence on hydration of acrylonitrile Nocardia sp.
4.2.1.84 6 10 hydration of acrylonitrile, pH Nocardia sp.